C57BL/6JCya-Stumem1/Cya
Common Name:
Stum-KO
Product ID:
S-KO-09741
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Stum-KO
Strain ID
KOCMP-381310-Stum-B6J-VA
Gene Name
Product ID
S-KO-09741
Gene Alias
6330403A02Rik; Gm10001
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Stumem1/Cya mice (Catalog S-KO-09741) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000136521
NCBI RefSeq
NM_001081227
Target Region
Exon 2
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Stum, or stumble, is an evolutionarily conserved gene essential for mechanical sensing in a specific subpopulation of Drosophila proprioceptive neurons that sense joint angles. The Stum protein has orthologs in animals from nematodes to mammals and is predicted to contain two transmembrane domains [1]. These neurons expressing Stum are crucial for animal locomotion as they generate proprioceptive feedback [1,3].
A genetic screen in Drosophila identified stum as a gene involved in mechanical transduction in leg joint proprioceptive neurons [1,3]. In these neurons, the dendrites are stretched by joint movements, leading to elevation of cellular Ca(2+) levels, which is absent in stum mutants [1]. Also, stum -expressing neurons express the mechanosensory channel NOMPC, and in nompC mutants, responses to joint position are abolished, indicating that NOMPC is the mechanosensitive channel in these neurons [3]. Additionally, a mutant initially thought to affect stum was later found to be a new allele of O -fucosyltransferase 1, acting through the Notch signaling pathway upstream of the S2 cleavage of the Notch receptor in Drosophila intestinal stem cell regulation [2].
In conclusion, Stum is vital for mechanical sensing in proprioceptive neurons, contributing to animal locomotion. The study of Stum in Drosophila models has revealed its role in mechanotransduction and potential connections to other signaling pathways like Notch, enhancing our understanding of the underlying biological processes related to proprioception and stem cell regulation.
References:
1. Desai, Bela S, Chadha, Abhishek, Cook, Boaz. . The stum gene is essential for mechanical sensing in proprioceptive neurons. In Science (New York, N.Y.), 343, 1256-9. doi:10.1126/science.1247761. https://pubmed.ncbi.nlm.nih.gov/24626929/
2. Shi, Lin, Kong, Ruiyan, Li, Zhengran, Li, Jing, Li, Zhouhua. 2021. Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation. In Biology open, 10, . doi:10.1242/bio.058910. https://pubmed.ncbi.nlm.nih.gov/34731235/
3. Chadha, Abhishek, Kaneko, Maki, Cook, Boaz. 2015. NOMPC-dependent mechanotransduction shapes the dendrite of proprioceptive neurons. In Neuroscience letters, 597, 111-6. doi:10.1016/j.neulet.2015.04.033. https://pubmed.ncbi.nlm.nih.gov/25916878/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen