C57BL/6JCya-Gpr25em1/Cya
Common Name:
Gpr25-KO
Product ID:
S-KO-09887
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gpr25-KO
Strain ID
KOCMP-383563-Gpr25-B6J-VA
Gene Name
Product ID
S-KO-09887
Gene Alias
Gm1300
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpr25em1/Cya mice (Catalog S-KO-09887) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000086395
NCBI RefSeq
NM_001101516
Target Region
Exon 1
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Gpr25, an orphan G protein-coupled receptor in vertebrates, has been implicated in autoimmune diseases and blood pressure regulation in humans [2]. It has been identified as a lymphocyte receptor for CXCL17, a chemoattractant cytokine. This forms a GPR25-CXCL17 chemoaffinity axis that may integrate immunity and tolerance at non-intestinal mucosae and the CNS [1]. Gpr25 can also be activated by Apelin and Apela in non-mammalian vertebrates, leading to the inhibition of forskolin-stimulated cAMP production [2].
Single-cell transcriptomic analyses show that Gpr25 is induced on innate lymphocytes before emigration to the periphery, and is imprinted in secondary lymphoid tissues on activated B and T cells in response to immune challenge. In mouse models, Gpr25 mediates lymphocyte homing to barrier epithelia of the airways, oral cavity, stomach, and biliary and genitourinary tracts, suggesting its role in non-intestinal mucosal immunity [1].
In conclusion, Gpr25 plays a significant role in the immune system, particularly in non-intestinal mucosal and CNS immunity. Its identification as a receptor for CXCL17 and its activation by Apelin and Apela in non-mammalian vertebrates provide important insights into its function. The study of Gpr25 in mouse models helps to understand its role in immune-related processes, potentially contributing to the understanding of autoimmune diseases and immune regulation in non-intestinal mucosal tissues and the CNS.
References:
1. Ocón, Borja, Xiang, Menglan, Bi, Yuhan, Pan, Junliang, Butcher, Eugene C. 2024. A lymphocyte chemoaffinity axis for lung, non-intestinal mucosae and CNS. In Nature, 635, 736-745. doi:10.1038/s41586-024-08043-2. https://pubmed.ncbi.nlm.nih.gov/39293486/
2. Zhang, Jiannan, Wan, Yiping, Fang, Chao, Li, Juan, Wang, Yajun. 2018. The orphan G protein-coupled receptor 25 (GPR25) is activated by Apelin and Apela in non-mammalian vertebrates. In Biochemical and biophysical research communications, 501, 408-414. doi:10.1016/j.bbrc.2018.04.229. https://pubmed.ncbi.nlm.nih.gov/29727602/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen