C57BL/6NCya-Banf2em1/Cya
Common Name:
Banf2-KO
Product ID:
S-KO-09975
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Banf2-KO
Strain ID
KOCMP-403171-Banf2-B6N-VA
Gene Name
Product ID
S-KO-09975
Gene Alias
4930517K23Rik; Baf-L; Baf-like; Gm115
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Banf2em1/Cya mice (Catalog S-KO-09975) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000037722
NCBI RefSeq
NM_207275
Target Region
Exon 3~4
Size of Effective Region
~9.4 kb
Detailed Document
Overview of Gene Research
Banf2, or barrier-to-autointegration factor 2, is a paralog of the widely-expressed barrier-to-autointegration factor (BAF). BAF is involved in chromatin organization, nuclear envelope assembly, gonadal development, and embryonic stem cell self-renewal [3,4,5]. Banf2 exhibits a testis-predominant expression pattern in humans and mice, and has been associated with male infertility in some studies, suggesting its potential role in spermatogenesis [1,2]. Genetic models, such as gene knockout mouse models, are valuable tools for studying its function.
Surprisingly, when researchers used the Nuclease technology system to generate Banf2-knockout mice, the knockout mice did not show any detectable abnormalities in the histological structure of the testis, epididymis, ovary, and other tissues, and had normal fertility. This indicates that Banf2 is not essential for mouse spermatogenesis and fertility [1]. However, transcriptomic data analysis in male infertility research showed that Banf2 was among the genes commonly differentially expressed between non-obstructive azoospermia (NOA) and non-obstructive and obstructive azoospermia (NOA and OA) groups. These genes are involved in germ cell development, spermatid development, spermatid differentiation, regulation of proteolysis, spermatogenesis, and metabolic processes, suggesting a potential link to male infertility [2].
In conclusion, while Banf2-knockout mouse models suggest that it is not essential for mouse spermatogenesis and fertility, transcriptomic data analysis implies its possible role in male infertility-related biological processes. The study of Banf2 using gene knockout models provides insights into its function, especially in the context of male reproductive health.
References:
1. Niu, Chang-Min, Xia, Meng-Meng, Zhong, Ya-Nan, Zheng, Ying. 2021. Mus musculus Barrier-To-Autointegration Factor 2 (Banf2) is Not Essential for Spermatogenesis or Fertility. In Cytogenetic and genome research, 161, 167-177. doi:10.1159/000513850. https://pubmed.ncbi.nlm.nih.gov/33951625/
2. Omolaoye, Temidayo S, Omolaoye, Victor A, Kandasamy, Richard K, Hachim, Mahmood Yaseen, Du Plessis, Stefan S. 2022. Omics and Male Infertility: Highlighting the Application of Transcriptomic Data. In Life (Basel, Switzerland), 12, . doi:10.3390/life12020280. https://pubmed.ncbi.nlm.nih.gov/35207567/
3. Margalit, Ayelet, Brachner, Andreas, Gotzmann, Josef, Foisner, Roland, Gruenbaum, Yosef. 2007. Barrier-to-autointegration factor--a BAFfling little protein. In Trends in cell biology, 17, 202-8. doi:. https://pubmed.ncbi.nlm.nih.gov/17320395/
4. Marcelot, Agathe, Worman, Howard J, Zinn-Justin, Sophie. 2020. Protein structural and mechanistic basis of progeroid laminopathies. In The FEBS journal, 288, 2757-2772. doi:10.1111/febs.15526. https://pubmed.ncbi.nlm.nih.gov/32799420/
5. Halfmann, Charles T, Roux, Kyle J. 2021. Barrier-to-autointegration factor: a first responder for repair of nuclear ruptures. In Cell cycle (Georgetown, Tex.), 20, 647-660. doi:10.1080/15384101.2021.1892320. https://pubmed.ncbi.nlm.nih.gov/33678126/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen