C57BL/6JCya-Tmprss2em1/Cya
Common Name:
Tmprss2-KO
Product ID:
S-KO-10194
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tmprss2-KO
Strain ID
KOCMP-50528-Tmprss2-B6J-VA
Gene Name
Product ID
S-KO-10194
Gene Alias
D16Ertd61e
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmprss2em1/Cya mice (Catalog S-KO-10194) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000000395
NCBI RefSeq
NM_015775.2
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Tmprss2, short for transmembrane serine protease 2, is a type II transmembrane protease encoded by the TMPRSS2 gene. It has two autocatalytically activated isoforms from its inactive zymogen form. Functionally, it is involved in priming viral spike proteins, facilitating viral entry and thus viral infectivity. It also has been associated with the renin-angiotensin system and may play a role in various biological processes in epithelial cells of the respiratory, gastrointestinal tract, and prostate [3,4,5,6,7].
Regarding its role in diseases, Tmprss2 has been extensively studied in relation to viral infections and cancer. In the context of viral infections, it serves as a major cofactor in SARS-CoV-2 entry, and primes glycoproteins of other respiratory viruses like human coronavirus HKU1 [3,4,6]. HKU1 uses both sialoglycan and Tmprss2 for cellular entry, where sialoglycan primes the spike protein for Tmprss2 binding, and Tmprss2 undergoes autocleavage to enhance its affinity for the HKU1 spike [2]. In cancer, a fusion gene between TMPRSS2 and ERG (an oncogenic transcription factor) is a common chromosomal aberration in prostate cancer, driving carcinogenesis [7]. Depletion of Tmprss2 in lung cancer cells suppressed anchorage-independent growth and xenograft tumor growth in mice, indicating its role in lung cancer progression as PM2.5 activates AhR to upregulate Tmprss2 and IL18 expression [1].
In conclusion, Tmprss2 is crucial in both viral infectivity and cancer-related processes. Its role in facilitating viral entry for various coronaviruses and its association with cancer development, as revealed through in vivo studies (although no KO/CKO mouse models were specifically detailed in the provided references), highlight its potential as a therapeutic target for treating viral infections and certain cancers.
References:
1. Wang, Tong-Hong, Huang, Kuo-Yen, Chen, Chin-Chuan, Yang, Pan-Chyr, Chen, Chi-Yuan. 2023. PM2.5 promotes lung cancer progression through activation of the AhR-TMPRSS2-IL18 pathway. In EMBO molecular medicine, 15, e17014. doi:10.15252/emmm.202217014. https://pubmed.ncbi.nlm.nih.gov/36975376/
2. Pan, Zhengyang, Li, Daoqun, Zhang, Leiliang. 2024. TMPRSS2 in microbial interactions: Insights from HKU1 and TcsH. In PLoS pathogens, 20, e1012677. doi:10.1371/journal.ppat.1012677. https://pubmed.ncbi.nlm.nih.gov/39565778/
3. Wettstein, Lukas, Kirchhoff, Frank, Münch, Jan. 2022. The Transmembrane Protease TMPRSS2 as a Therapeutic Target for COVID-19 Treatment. In International journal of molecular sciences, 23, . doi:10.3390/ijms23031351. https://pubmed.ncbi.nlm.nih.gov/35163273/
4. Dong, Mengzhen, Zhang, Jie, Ma, Xuefeng, Xin, Yongning, Zhuang, Likun. 2020. ACE2, TMPRSS2 distribution and extrapulmonary organ injury in patients with COVID-19. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 131, 110678. doi:10.1016/j.biopha.2020.110678. https://pubmed.ncbi.nlm.nih.gov/32861070/
5. Zipeto, Donato, Palmeira, Julys da Fonseca, Argañaraz, Gustavo A, Argañaraz, Enrique R. 2020. ACE2/ADAM17/TMPRSS2 Interplay May Be the Main Risk Factor for COVID-19. In Frontiers in immunology, 11, 576745. doi:10.3389/fimmu.2020.576745. https://pubmed.ncbi.nlm.nih.gov/33117379/
6. Abbasi, Asim Z, Kiyani, Dania A, Hamid, Syeda M, Fahim, Ammad, Jalal, Nasir. 2021. Spiking dependence of SARS-CoV-2 pathogenicity on TMPRSS2. In Journal of medical virology, 93, 4205-4218. doi:10.1002/jmv.26911. https://pubmed.ncbi.nlm.nih.gov/33638460/
7. Thunders, Michelle, Delahunt, Brett. 2020. Gene of the month: TMPRSS2 (transmembrane serine protease 2). In Journal of clinical pathology, 73, 773-776. doi:10.1136/jclinpath-2020-206987. https://pubmed.ncbi.nlm.nih.gov/32873700/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen