C57BL/6JCya-Rcn3em1/Cya
Common Name
Rcn3-KO
Product ID
S-KO-10312
Backgroud
C57BL/6JCya
Strain ID
KOCMP-52377-Rcn3-B6J-VA
Status
When using this mouse strain in a publication, please cite “Rcn3-KO Mouse (Catalog S-KO-10312) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Rcn3-KO
Strain ID
KOCMP-52377-Rcn3-B6J-VA
Gene Name
Product ID
S-KO-10312
Gene Alias
RLP49, D7Ertd671e, 6030455P07Rik, D530026G20Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 7
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000019683
NCBI RefSeq
NM_026555
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Overview of Gene Research
Rcn3, also known as Reticulocalbin 3, is an endoplasmic reticulum (ER) lumen-residing Ca2+ binding protein belonging to the CREC family. It is located in the secretory pathway of living cells and plays an essential role in protein biosynthesis processes via this pathway [2,3].
In vivo studies using gene knockout models have revealed significant functions of Rcn3. In a fibroblast-selective knockdown mouse model, repressing Rcn3 expression ameliorated bleomycin-induced lung fibrosis and pulmonary dysfunction. Mechanistically, RCN3 promotes fibroblast activation through a TGFβ1-RCN3-TGFBR1 positive feedback loop, upregulating TGFBR1 expression [1]. In a type II alveolar epithelial cell (AECIIs) specific Rcn3 conditional knockout (CKO) mouse model, the selective ablation of Rcn3 in AECIIs significantly alleviated the severity of porcine pancreatic elastase-induced emphysema [4].
In conclusion, Rcn3 is crucial in protein biosynthesis and is involved in regulating processes related to lung-associated diseases. The Rcn3 knockout and CKO mouse models have been instrumental in uncovering its role in pulmonary fibrosis and emphysema, providing insights into potential therapeutic targets for these diseases.
References:
1. Wu, Mingting, Wang, Zhenyan, Shi, Xiaoqian, Ma, Yingmin, Jin, Jiawei. 2023. TGFβ1-RCN3-TGFBR1 loop facilitates pulmonary fibrosis by orchestrating fibroblast activation. In Respiratory research, 24, 222. doi:10.1186/s12931-023-02533-z. https://pubmed.ncbi.nlm.nih.gov/37710230/
2. Hou, Yu, Li, Yongchao, Gong, Fuxing, Fang, Qiuhong, Ma, Runlin Z. . A Preliminary Study on RCN3 Protein Expression in Non-small Cell Lung Cancer. In Clinical laboratory, 62, 293-300. doi:. https://pubmed.ncbi.nlm.nih.gov/27156316/
3. He, Yi, Alejo, Salvador, Johnson, Jessica D, Jayamohan, Sridharan, Sareddy, Gangadhara R. 2023. Reticulocalbin 3 Is a Novel Mediator of Glioblastoma Progression. In Cancers, 15, . doi:10.3390/cancers15072008. https://pubmed.ncbi.nlm.nih.gov/37046668/
4. Zhang, Qianyu, Wang, Tong, Jin, Jiawei, Z Ma, Runlin, Fang, Qiuhong. 2021. Rcn3 Suppression Was Responsible for Partial Relief of Emphysema as Shown by Specific Type II Alveolar Epithelial Cell Rcn3 CKO Mouse Model. In International journal of chronic obstructive pulmonary disease, 16, 147-158. doi:10.2147/COPD.S272711. https://pubmed.ncbi.nlm.nih.gov/33531801/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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