C57BL/6JCya-Mtg2em1/Cya
Common Name
Mtg2-KO
Product ID
S-KO-10368
Backgroud
C57BL/6JCya
Strain ID
KOCMP-52856-Mtg2-B6J-VA
Status
When using this mouse strain in a publication, please cite “Mtg2-KO Mouse (Catalog S-KO-10368) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Mtg2-KO
Strain ID
KOCMP-52856-Mtg2-B6J-VA
Gene Name
Product ID
S-KO-10368
Gene Alias
Gtpbp5, D2Bwg0647e, 1810011P19Rik, 2900056P18Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000108901
NCBI RefSeq
NM_001083329
Target Region
Exon 2~7
Size of Effective Region
~8.2 kb
Overview of Gene Research
Mtg2, also known as GTPBP5, is a mitochondrial protein belonging to the Obg family. It plays a crucial role in the biogenesis of mammalian mitochondrial ribosomes (mitoribosomes), specifically facilitating the maturation of the large mitoribosome subunit (mtLSU) by ensuring efficient methylation of two 16S rRNA residues [2]. This ultimately coordinates subunit joining, serving as a quality control checkpoint during mtLSU assembly to ensure the formation of the mature 55S monosome.
In a study on Asian neovascular age-related macular degeneration (nAMD) subtypes, at the GATA binding protein 5 (GATA5) locus, the expressions of genes including Mtg2 were significantly induced during retinal angiogenesis and subretinal fibrosis in murine models [1]. Also, in the filamentous fungus Botrytis cinerea, the ortholog BcMtg2 is required for asexual development, environmental stress response, and pathogenicity as its deletion mutant showed defects in spore production, conidial germination, sclerotial formation, and increased sensitivity to various environmental stresses [3].
In conclusion, Mtg2 is essential for mitoribosome maturation, which has implications for normal cellular function. Its altered expression in models related to nAMD and its role in the development and stress response of Botrytis cinerea suggest its importance in both mammalian disease-related processes and fungal biology. The study of Mtg2 using gene knockout models in these contexts provides insights into disease mechanisms and fungal pathogenicity.
References:
1. Fan, Qiao, Li, Hengtong, Wang, Xiaomeng, Cheung, Chui Ming Gemmy, Cheng, Ching-Yu. 2023. Contribution of common and rare variants to Asian neovascular age-related macular degeneration subtypes. In Nature communications, 14, 5574. doi:10.1038/s41467-023-41256-z. https://pubmed.ncbi.nlm.nih.gov/37696869/
2. Maiti, Priyanka, Antonicka, Hana, Gingras, Anne-Claude, Shoubridge, Eric A, Barrientos, Antoni. . Human GTPBP5 (MTG2) fuels mitoribosome large subunit maturation by facilitating 16S rRNA methylation. In Nucleic acids research, 48, 7924-7943. doi:10.1093/nar/gkaa592. https://pubmed.ncbi.nlm.nih.gov/32652011/
3. Shao, Wenyong, Zhang, Yu, Wang, Jin, Lv, Chiyuan, Chen, Changjun. 2016. BcMtg2 is required for multiple stress tolerance, vegetative development and virulence in Botrytis cinerea. In Scientific reports, 6, 28673. doi:10.1038/srep28673. https://pubmed.ncbi.nlm.nih.gov/27346661/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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