C57BL/6JCya-Atp8b1em1/Cya
Common Name:
Atp8b1-KO
Product ID:
S-KO-10620
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Atp8b1-KO
Strain ID
KOCMP-54670-Atp8b1-B6J-VA
Gene Name
Product ID
S-KO-10620
Gene Alias
FIC1; Ic
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Atp8b1em1/Cya mice (Catalog S-KO-10620) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025482
NCBI RefSeq
NM_001001488
Target Region
Exon 3~8
Size of Effective Region
~9.8 kb
Detailed Document
Overview of Gene Research
Atp8b1, encoding FIC1, is a P4-ATPase phospholipid flippase. It's crucial for maintaining phospholipid asymmetry in biological membranes, which is essential for membrane protein targeting, vesicle biogenesis, and barrier function [3,5]. It's involved in multiple pathways such as lysosomal fusion in macrophages, and has potential connections to diseases like cholestasis [4,5]. Genetic models, like knockout mice, have been valuable in studying its functions.
In intestinal epithelial cell-specific Atp8b1-knockout (Atp8b1IEC-KO) mice, loss of Atp8b1 leads to malabsorption of lysophosphatidylcholine (LPC), causing hepatic choline deficiency and progression to steatohepatitis by 4 weeks. However, choline-supplemented diets can reverse this condition, indicating Atp8b1 regulates hepatic choline levels via intestinal LPC absorption [1]. In pancreatic acinar cells, during chronic pancreatitis development in PRSS1 transgenic mice, decreased Atp8b1 expression was observed. Atp8b1 complementation increased LPC concentration and improved disease outcomes, suggesting the acinar Atp8b1/LPC pathway has a protective role [2].
In conclusion, Atp8b1 plays essential roles in maintaining membrane phospholipid asymmetry and is involved in processes like lysosomal fusion, and regulation of hepatic choline levels. Gene-knockout mouse models have revealed its significance in diseases such as steatohepatitis and chronic pancreatitis, providing insights into potential therapeutic strategies for these conditions.
References:
1. Tamura, Ryutaro, Sabu, Yusuke, Mizuno, Tadahaya, Ando, Tomohiro, Hayashi, Hisamitsu. 2023. Intestinal Atp8b1 dysfunction causes hepatic choline deficiency and steatohepatitis. In Nature communications, 14, 6763. doi:10.1038/s41467-023-42424-x. https://pubmed.ncbi.nlm.nih.gov/37990006/
2. Yang, Wan-Jun, Cao, Rong-Chang, Xiao, Wang, Jin, Jin, Zhang, Guo-Wei. 2022. Acinar ATP8b1/LPC pathway promotes macrophage efferocytosis and clearance of inflammation during chronic pancreatitis development. In Cell death & disease, 13, 893. doi:10.1038/s41419-022-05322-6. https://pubmed.ncbi.nlm.nih.gov/36273194/
3. Dieudonné, Thibaud, Kümmerer, Felix, Laursen, Michelle Juknaviciute, Lindorff-Larsen, Kresten, Nissen, Poul. 2023. Activation and substrate specificity of the human P4-ATPase ATP8B1. In Nature communications, 14, 7492. doi:10.1038/s41467-023-42828-9. https://pubmed.ncbi.nlm.nih.gov/37980352/
4. Bull, Laura N, Thompson, Richard J. 2018. Progressive Familial Intrahepatic Cholestasis. In Clinics in liver disease, 22, 657-669. doi:10.1016/j.cld.2018.06.003. https://pubmed.ncbi.nlm.nih.gov/30266155/
5. Gómez-Mellado, Valentina E, Ho-Mok, Kam S, van der Mark, Vincent A, Elferink, Ronald P J Oude, Paulusma, Coen C. 2022. The phospholipid flippase ATP8B1 is required for lysosomal fusion in macrophages. In Cell biochemistry and function, 40, 914-925. doi:10.1002/cbf.3752. https://pubmed.ncbi.nlm.nih.gov/36169099/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen