C57BL/6JCya-Eif3dem1/Cya
Common Name:
Eif3d-KO
Product ID:
S-KO-10657
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Eif3d-KO
Strain ID
KOCMP-55944-Eif3d-B6J-VA
Gene Name
Product ID
S-KO-10657
Gene Alias
66/67kDa; Eif3s7; eIF3p66
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Eif3dem1/Cya mice (Catalog S-KO-10657) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000100484
NCBI RefSeq
NM_018749
Target Region
Exon 4~12
Size of Effective Region
~5.8 kb
Detailed Document
Overview of Gene Research
Eif3d, eukaryotic translation initiation factor 3d, is an RNA-binding subunit of the eIF3 complex. It has an RNA-binding motif and a cap-binding domain. Eif3d is involved in maintaining eIF3 complex integrity and global protein synthesis. It also plays a role in non-canonical cap-dependent translation of specific mRNAs, which is crucial for various biological and pathological processes [2].
During persistent stress, eIF3d activates the translation of the kinase GCN2, inducing eIF2α phosphorylation and inhibiting general protein synthesis. In parallel, it upregulates the m6A demethylase ALKBH5 to drive 5' UTR-specific demethylation of stress response genes like ATF4, ultimately controlling the integrated stress response (ISR) [1]. In cervical carcinoma, EIF3D promotes cell growth and the Warburg effect by interacting with GRP78 [3]. In colorectal cancer, EIF3D promotes resistance to 5-fluorouracil through upregulating RUVBL1 [4].
In conclusion, Eif3d is essential for regulating translation processes and has significant implications in stress responses and multiple diseases such as cervical carcinoma and colorectal cancer. Studies on Eif3d, especially through loss-of-function experiments, help in understanding its role in these biological processes and provide potential therapeutic targets for related diseases.
References:
1. Mukhopadhyay, Shaoni, Amodeo, Maria E, Lee, Amy S Y. 2023. eIF3d controls the persistent integrated stress response. In Molecular cell, 83, 3303-3313.e6. doi:10.1016/j.molcel.2023.08.008. https://pubmed.ncbi.nlm.nih.gov/37683648/
2. Ma, Shijie, Liu, Jing-Yuan, Zhang, Jian-Ting. 2023. eIF3d: A driver of noncanonical cap-dependent translation of specific mRNAs and a trigger of biological/pathological processes. In The Journal of biological chemistry, 299, 104658. doi:10.1016/j.jbc.2023.104658. https://pubmed.ncbi.nlm.nih.gov/36997088/
3. Liu, Qing, Liu, Jing, Zheng, Dan, Zhou, Xiaochan, Qin, Juan. 2022. EIF3D promoted cervical carcinoma through Warburg effect by interacting with GRP78. In Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology, 43, 2130200. doi:10.1080/01443615.2022.2130200. https://pubmed.ncbi.nlm.nih.gov/36264610/
4. Li, Chaobin, Lu, Kemei, Yang, Chenggang, Du, Wenfeng, Liang, Zhengkai. 2023. EIF3D promotes resistance to 5-fluorouracil in colorectal cancer through upregulating RUVBL1. In Journal of clinical laboratory analysis, 37, e24825. doi:10.1002/jcla.24825. https://pubmed.ncbi.nlm.nih.gov/36592991/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen