C57BL/6NCya-Snx1em1/Cya
Common Name:
Snx1-KO
Product ID:
S-KO-10823
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Snx1-KO
Strain ID
KOCMP-56440-Snx1-B6N-VA
Gene Name
Product ID
S-KO-10823
Gene Alias
--
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Snx1em1/Cya mice (Catalog S-KO-10823) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034946
NCBI RefSeq
NM_019727
Target Region
Exon 2~4
Size of Effective Region
~4.8 kb
Detailed Document
Overview of Gene Research
Snx1, a member of the sorting nexin (SNX) family, is crucial for membrane remodeling and endosomal transport [1,2,5]. It has a role in generating transport carriers in endosomal pathways, and its functions are associated with the retromer complex and endosomal SNX-BAR sorting complex for promoting exit 1 complex trafficking [1,2]. Genetic models like knockout mice can provide insights into its specific functions.
In Parkinson's disease models, dysregulation of the SNX1-retromer axis was observed. Stagnation of retromer-mediated retrograde transport occurred in different PD-mimetic conditions. DNAJC13 was involved in clathrin-dependent retromer transport as a functional modulator of SNX1, and excess α-synuclein decreased the interaction between SNX1 and VPS35, a core component of retromer. R33, a pharmacological retromer chaperone, reduced insoluble α-synuclein and mitigated rotenone-induced neuronal apoptosis, suggesting SNX1-retromer-regulated retrograde transport may be involved in PD pathogenesis [4]. In gefitinib-resistant NSCLC cell lines, overexpressed Snx1 was associated with impaired EGFR endocytosis, where internalized EGFR aggregated in early endosomes, implicating dysregulated EGFR endocytosis in gefitinib resistance [3].
In conclusion, Snx1 is essential for endosomal membrane remodeling and transport. Through model-based research, its role in diseases like Parkinson's disease and NSCLC drug resistance has been revealed. These findings highlight Snx1 as a potential target for disease-modifying therapies in these disease areas.
References:
1. Zhang, Yan, Pang, Xiaoyun, Li, Jian, Hsu, Victor W, Sun, Fei. . Structural insights into membrane remodeling by SNX1. In Proceedings of the National Academy of Sciences of the United States of America, 118, . doi:10.1073/pnas.2022614118. https://pubmed.ncbi.nlm.nih.gov/33658379/
2. Da Graça, Juliane, Morel, Etienne. 2023. Canonical and Non-Canonical Roles of SNX1 and SNX2 in Endosomal Membrane Dynamics. In Contact (Thousand Oaks (Ventura County, Calif.)), 6, 25152564231217867. doi:10.1177/25152564231217867. https://pubmed.ncbi.nlm.nih.gov/38033809/
3. Nishimura, Yukio, Itoh, Kazuyuki. 2019. Involvement of SNX1 in regulating EGFR endocytosis in a gefitinib-resistant NSCLC cell lines. In Cancer drug resistance (Alhambra, Calif.), 2, 539-549. doi:10.20517/cdr.2019.15. https://pubmed.ncbi.nlm.nih.gov/35582586/
4. Yoshida, Shun, Hasegawa, Takafumi, Nakamura, Takaaki, Takeda, Atsushi, Aoki, Masashi. 2024. Dysregulation of SNX1-retromer axis in pharmacogenetic models of Parkinson's disease. In Cell death discovery, 10, 290. doi:10.1038/s41420-024-02062-8. https://pubmed.ncbi.nlm.nih.gov/38886344/
5. Yong, Xin, Hu, Wenfeng, Zhou, Xue, Burstein, Ezra, Jia, Da. 2018. Expression and purification of the SNX1/SNX6 complex. In Protein expression and purification, 151, 93-98. doi:10.1016/j.pep.2018.06.010. https://pubmed.ncbi.nlm.nih.gov/29908913/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen