C57BL/6JCya-Dnase2bem1/Cya
Common Name:
Dnase2b-KO
Product ID:
S-KO-10891
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dnase2b-KO
Strain ID
KOCMP-56629-Dnase2b-B6J-VA
Gene Name
Product ID
S-KO-10891
Gene Alias
Dlad; DnaseIIb
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dnase2bem1/Cya mice (Catalog S-KO-10891) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000200633
NCBI RefSeq
NM_019957
Target Region
Exon 3~6
Size of Effective Region
~9.1 kb
Detailed Document
Overview of Gene Research
Dnase2b, the ancestral gene of the DNase2 family, has been conserved in synteny with the Uox gene across 700 million years of animal evolution. It is an enzyme with a key function in nuclear degradation, especially in the mouse lens [1,2]. In the context of lens development, this function is crucial as the lens needs to undergo organelle and nuclear degradation to become transparent, suggesting its importance in maintaining tissue transparency and proper development [2,3].
In Celf1-targeted knockout mice, the RNA-binding protein Celf1 directly binds to the mRNA of Dnase2b to maintain its high levels. This event is necessary for Cdk1-mediated lamin A/C phosphorylation to initiate nuclear envelope breakdown and DNA degradation in fiber cells. Celf1 deficiency leads to ectopic up-regulation of p21Cip1 and down-regulation of Dnase2b, resulting in severe eye defects/cataract [2]. In Xenopus, inactivation of dnase2b by Nuclease technology produces lens defects similar to human cataracts, including nuclei retention in lens fiber cells and actin fibers disorganization [4].
In conclusion, Dnase2b plays an essential role in nuclear degradation, particularly in lens development. Findings from gene knockout models in mice and Xenopus have revealed its significance in preventing cataract-like phenotypes. These studies highlight the importance of Dnase2b in maintaining proper lens function and offer insights into the molecular mechanisms underlying cataract formation [2,4].
References:
1. Mori, Giulia, Delfino, Danila, Pibiri, Paola, Rivetti, Claudio, Percudani, Riccardo. 2022. Origin and significance of the human DNase repertoire. In Scientific reports, 12, 10364. doi:10.1038/s41598-022-14133-w. https://pubmed.ncbi.nlm.nih.gov/35725583/
2. Siddam, Archana D, Gautier-Courteille, Carole, Perez-Campos, Linette, Paillard, Luc, Lachke, Salil A. 2018. The RNA-binding protein Celf1 post-transcriptionally regulates p27Kip1 and Dnase2b to control fiber cell nuclear degradation in lens development. In PLoS genetics, 14, e1007278. doi:10.1371/journal.pgen.1007278. https://pubmed.ncbi.nlm.nih.gov/29565969/
3. Iida, Atsumi, Tabata, Yoko, Baba, Yukihiro, Fujii, Tomoaki, Watanabe, Sumiko. 2014. Critical roles of DNase1l3l in lens nuclear degeneration in zebrafish. In Biochimie, 106, 68-74. doi:10.1016/j.biochi.2014.07.025. https://pubmed.ncbi.nlm.nih.gov/25127661/
4. Viet, Justine, Reboutier, David, Hardy, Serge, Paillard, Luc, Gautier-Courteille, Carole. 2020. Modeling ocular lens disease in Xenopus. In Developmental dynamics : an official publication of the American Association of Anatomists, 249, 610-621. doi:10.1002/dvdy.147. https://pubmed.ncbi.nlm.nih.gov/31872467/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen