Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6NCya-Dnajb8em1/Cya
Common Name:
Dnajb8-KO
Product ID:
S-KO-10903
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Dnajb8-KO
Strain ID
KOCMP-56691-Dnajb8-B6N-VA
Gene Name
Dnajb8
Product ID
S-KO-10903
Gene Alias
1700016F14Rik; 1700031F10Rik; mDj6
Background
C57BL/6NCya
NCBI ID
56691
Modification
Conventional knockout
Chromosome
6
Phenotype
MGI:1922801
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Dnajb8em1/Cya mice (Catalog S-KO-10903) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000061866
NCBI RefSeq
NM_019964
Target Region
Exon 1
Size of Effective Region
~0.9 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Dnajb8, a member of the heat shock protein 40 (HSP40) family, is a J-domain protein (JDP) molecular chaperone. JDPs are central in maintaining a healthy proteome. Dnajb8 is involved in various cellular processes such as protein folding, and it may interact with pathways related to stress response, autophagy, and drug resistance [1,2,3,4]. Genetic models, like knockout mice, can be valuable in studying its functions.

In a knockout (KO) mouse model, Dnajb8 was found dispensable for spermatogenesis and male fertility [5]. In renal cell carcinoma cell lines, Dnajb8 KO cells showed reduced side population cells, decreased sphere-forming ability, and increased sensitivity to Docetaxel, indicating its role in tumor initiation and drug resistance [6]. Treatment with siRNA targeting Dnajb8 restored Oxaliplatin sensitivity in colon cancer cells in vitro and in vivo, as Dnajb8 promotes Oxaliplatin resistance through the TP53/MDR1 pathway [2]. DNAJB8 also restricts HIV-1 virion infectivity by facilitating autophagic-lysosomal degradation of viral Vif protein and rescuing APOBEC3G expression [3]. It inhibits pseudorabies virus replication by promoting cellular autophagy [4].

In conclusion, Dnajb8 plays diverse roles in biological processes. Model-based research, especially KO mouse models, has revealed its significance in cancer drug resistance, viral infections, and male fertility-related processes. Understanding Dnajb8's functions provides insights into disease mechanisms and potential therapeutic targets for conditions like colon cancer, HIV-1 infection, and pseudorabies virus-related diseases.

References:

1. Ryder, Bryan D, Ustyantseva, Elizaveta, Boyer, David R, Kampinga, Harm H, Joachimiak, Lukasz A. 2024. DNAJB8 oligomerization is mediated by an aromatic-rich motif that is dispensable for substrate activity. In Structure (London, England : 1993), 32, 662-678.e8. doi:10.1016/j.str.2024.02.015. https://pubmed.ncbi.nlm.nih.gov/38508190/

2. Wang, Zheng, Li, Yi, Mao, Rui, Liu, Yanjun, Zhang, Tongtong. 2022. DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer. In Cell death & disease, 13, 151. doi:10.1038/s41419-022-04599-x. https://pubmed.ncbi.nlm.nih.gov/35165262/

3. Chand, Kailash, Barman, Muneesh Kumar, Ghosh, Payel, Mitra, Debashis. . DNAJB8 facilitates autophagic-lysosomal degradation of viral Vif protein and restricts HIV-1 virion infectivity by rescuing APOBEC3G expression in host cells. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 37, e22793. doi:10.1096/fj.202201738R. https://pubmed.ncbi.nlm.nih.gov/36723955/

4. Chen, Xiaoyong, Li, Wenfeng. 2024. The heat shock protein DNAJB8 inhibits pseudorabies virus replication by autophagy. In Veterinary microbiology, 295, 110165. doi:10.1016/j.vetmic.2024.110165. https://pubmed.ncbi.nlm.nih.gov/38936156/

5. Wang, Fengsong, Kong, Shuai, Hu, Xuechun, Ye, Lan, Bai, Shun. 2020. Dnajb8, a target gene of SOX30, is dispensable for male fertility in mice. In PeerJ, 8, e10582. doi:10.7717/peerj.10582. https://pubmed.ncbi.nlm.nih.gov/33391882/

6. Yamashita, Masamichi, Hirohashi, Yoshihiko, Torigoe, Toshihiko, Imagawa, Tomohiro, Sato, Noriyuki. 2016. Dnajb8, a Member of the Heat Shock Protein 40 Family Has a Role in the Tumor Initiation and Resistance to Docetaxel but Is Dispensable for Stress Response. In PloS one, 11, e0146501. doi:10.1371/journal.pone.0146501. https://pubmed.ncbi.nlm.nih.gov/26751205/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest