C57BL/6JCya-Namptem1/Cya
Common Name:
Nampt-KO
Product ID:
S-KO-11160
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Nampt-KO
Strain ID
KOCMP-59027-Nampt-B6J-VA
Gene Name
Product ID
S-KO-11160
Gene Alias
1110035O14Rik; NAmPRTase; Pbef; Pbef1; Visfatin
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Namptem1/Cya mice (Catalog S-KO-11160) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020886
NCBI RefSeq
NM_021524
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Nicotinamide phosphoribosyltransferase (NAMPT), also known as visfatin, is a key regulator of the intracellular nicotinamide adenine dinucleotide (NAD) pool. NAD is an essential coenzyme in cellular redox reactions and a substrate for NAD-dependent enzymes. NAMPT catalyzes the rate-limiting step in NAD biosynthesis from nicotinamide, influencing the activity of NAD-dependent enzymes and regulating cellular metabolism. It is involved in multiple biological processes and is associated with various metabolic and age-related disorders [1,3,4,7].
In cancer, NAMPT over-expression is a common strategy for tumors to sustain NAD production. Inhibiting NAMPT can deplete NAD and impair cellular metabolism in cancer cells. For example, in ovarian cancer, NAMPT inhibition suppresses cancer stem-like cells associated with therapy-induced senescence, and a combination of NAMPT inhibitor FK866 and cisplatin improves the survival of EOC-bearing mice [2]. In non-small cell lung cancer, lncRNA ITGB2-AS1 promotes cisplatin resistance by activating the FOSL2/NAMPT axis [5]. In necrotizing enterocolitis, NAMPT inhibitor FK866 relieves intestinal inflammation by regulating macrophage activation [6].
In conclusion, NAMPT is crucial for maintaining NAD levels and regulating cellular metabolism. Model-based research, especially through NAMPT inhibition in in vivo studies, has revealed its significant roles in cancer, intestinal inflammation, and other disease conditions. Understanding NAMPT's functions provides potential therapeutic strategies for these diseases [2,5,6,7].
References:
1. Garten, Antje, Schuster, Susanne, Penke, Melanie, de Giorgis, Tommaso, Kiess, Wieland. 2015. Physiological and pathophysiological roles of NAMPT and NAD metabolism. In Nature reviews. Endocrinology, 11, 535-46. doi:10.1038/nrendo.2015.117. https://pubmed.ncbi.nlm.nih.gov/26215259/
2. Nacarelli, Timothy, Fukumoto, Takeshi, Zundell, Joseph A, Borowsky, Mark E, Zhang, Rugang. 2019. NAMPT Inhibition Suppresses Cancer Stem-like Cells Associated with Therapy-Induced Senescence in Ovarian Cancer. In Cancer research, 80, 890-900. doi:10.1158/0008-5472.CAN-19-2830. https://pubmed.ncbi.nlm.nih.gov/31857293/
3. Velma, Ganga Reddy, Krider, Isabella S, Alves, Erick T M, Laham, Megan S, Thatcher, Gregory R J. 2024. Channeling Nicotinamide Phosphoribosyltransferase (NAMPT) to Address Life and Death. In Journal of medicinal chemistry, 67, 5999-6026. doi:10.1021/acs.jmedchem.3c02112. https://pubmed.ncbi.nlm.nih.gov/38580317/
4. Ozgencil, Fikriye, Gunindi, Habibe Beyza, Eren, Gokcen. 2024. Dual-targeted NAMPT inhibitors as a progressive strategy for cancer therapy. In Bioorganic chemistry, 149, 107509. doi:10.1016/j.bioorg.2024.107509. https://pubmed.ncbi.nlm.nih.gov/38824699/
5. Chen, Huiyong, Wang, Linhui, Liu, Jingting, Liao, Hongliang, Wan, Renping. . LncRNA ITGB2-AS1 promotes cisplatin resistance of non-small cell lung cancer by inhibiting ferroptosis via activating the FOSL2/NAMPT axis. In Cancer biology & therapy, 24, 2223377. doi:10.1080/15384047.2023.2223377. https://pubmed.ncbi.nlm.nih.gov/37370246/
6. Liu, Qianyang, Gao, Kai, Ding, Xionghui, Chen, Gongli, Guo, Chunbao. 2023. NAMPT inhibition relieves intestinal inflammation by regulating macrophage activation in experimental necrotizing enterocolitis. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 165, 115012. doi:10.1016/j.biopha.2023.115012. https://pubmed.ncbi.nlm.nih.gov/37329710/
7. Gasparrini, Massimiliano, Giovannuzzi, Simone, Nocentini, Alessio, Raffaelli, Nadia, Supuran, Claudiu T. 2024. Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) in cancer: a patent review. In Expert opinion on therapeutic patents, 34, 565-582. doi:10.1080/13543776.2024.2367006. https://pubmed.ncbi.nlm.nih.gov/38861278/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen