Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Fads3em1/Cya
Common Name:
Fads3-KO
Product ID:
S-KO-11211
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Fads3-KO
Strain ID
KOCMP-60527-Fads3-B6J-VA
Gene Name
Fads3
Product ID
S-KO-11211
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
60527
Modification
Conventional knockout
Chromosome
19
Phenotype
MGI:1928740
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fads3em1/Cya mice (Catalog S-KO-11211) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000115995
NCBI RefSeq
NM_021890
Target Region
Exon 2~11
Size of Effective Region
~4.3 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Fads3, a member of the Fatty Acid Desaturase (FADS) gene cluster, was initially suspected to code for a new mammalian membrane-bound fatty acid desaturase due to its high nucleotide sequence homology with FADS1 and FADS2 [1,4]. It is involved in multiple lipid-related metabolic pathways. In the biosynthesis of sphingolipids, Fads3 is responsible for introducing a cis double bond in 4,14-sphingadiene (sphingadiene; SPD), and shows substrate specificity towards sphingosine-containing ceramides (SPH-CERs) [2]. It also functions as a Δ14Z sphingoid base desaturase, contributing to gender differences in the human plasma sphingolipidome by forming Δ14Z double-bond-containing long-chain bases (LCBs) such as d18:2 and m18:1 [3].

In a Fads3 knockout (KO) mouse model, docosahexaenoic acid (DHA, 22:6n-3) levels in the brain of postnatal day 1 (P1) KO mice were lower than in wild-type (WT) mice, and the ratio of docosapentaenoic acid (DPA, 22:5n-3) to DHA in P1 KO liver was higher, suggesting lower desaturase activity. Concomitantly, changes in other fatty acid levels and related gene expressions were observed, indicating that Fads3 may enhance liver-mediated 22:6n-3 synthesis to support brain 22:6n-3 accretion before and during the brain growth spurt [5].

In conclusion, Fads3 has diverse functions in lipid metabolism, playing a role in sphingolipid biosynthesis and influencing fatty acid composition in the liver and brain. The Fads3 KO mouse model has provided insights into its role in supporting brain DHA accretion, and further research on Fads3 may offer a better understanding of lipid-related biological processes and potentially related disease mechanisms [5].

References:

1. Rioux, Vincent, Legrand, Philippe. 2019. Fatty Acid Desaturase 3 (FADS3) Is a Specific ∆13-Desaturase of Ruminant trans-Vaccenic Acid. In Lifestyle genomics, 12, 18-24. doi:10.1159/000502356. https://pubmed.ncbi.nlm.nih.gov/32911476/

2. Jojima, Keisuke, Kihara, Akio. 2023. Metabolism of sphingadiene and characterization of the sphingadiene-producing enzyme FADS3. In Biochimica et biophysica acta. Molecular and cell biology of lipids, 1868, 159335. doi:10.1016/j.bbalip.2023.159335. https://pubmed.ncbi.nlm.nih.gov/37209771/

3. Karsai, Gergely, Lone, Museer, Kutalik, Zoltán, von Eckardstein, Arnold, Hornemann, Thorsten. 2019. FADS3 is a Δ14Z sphingoid base desaturase that contributes to gender differences in the human plasma sphingolipidome. In The Journal of biological chemistry, 295, 1889-1897. doi:10.1074/jbc.AC119.011883. https://pubmed.ncbi.nlm.nih.gov/31862735/

4. Blanchard, H, Legrand, P, Pédrono, F. 2010. Fatty Acid Desaturase 3 (Fads3) is a singular member of the Fads cluster. In Biochimie, 93, 87-90. doi:10.1016/j.biochi.2010.03.002. https://pubmed.ncbi.nlm.nih.gov/20226833/

5. Zhang, Ji Yao, Qin, Xia, Liang, Allison, Kothapalli, Kumar S D, Brenna, J Thomas. 2017. Fads3 modulates docosahexaenoic acid in liver and brain. In Prostaglandins, leukotrienes, and essential fatty acids, 123, 25-32. doi:10.1016/j.plefa.2017.07.001. https://pubmed.ncbi.nlm.nih.gov/28838557/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest