C57BL/6JCya-Fads3em1/Cya
Common Name:
Fads3-KO
Product ID:
S-KO-11211
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Fads3-KO
Strain ID
KOCMP-60527-Fads3-B6J-VA
Gene Name
Product ID
S-KO-11211
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fads3em1/Cya mice (Catalog S-KO-11211) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000115995
NCBI RefSeq
NM_021890
Target Region
Exon 2~11
Size of Effective Region
~4.3 kb
Detailed Document
Overview of Gene Research
Fads3, a member of the Fatty Acid Desaturase (FADS) gene cluster, was initially suspected to code for a new mammalian membrane-bound fatty acid desaturase due to its high nucleotide sequence homology with FADS1 and FADS2 [1,4]. It is involved in multiple lipid-related metabolic pathways. In the biosynthesis of sphingolipids, Fads3 is responsible for introducing a cis double bond in 4,14-sphingadiene (sphingadiene; SPD), and shows substrate specificity towards sphingosine-containing ceramides (SPH-CERs) [2]. It also functions as a Δ14Z sphingoid base desaturase, contributing to gender differences in the human plasma sphingolipidome by forming Δ14Z double-bond-containing long-chain bases (LCBs) such as d18:2 and m18:1 [3].
In a Fads3 knockout (KO) mouse model, docosahexaenoic acid (DHA, 22:6n-3) levels in the brain of postnatal day 1 (P1) KO mice were lower than in wild-type (WT) mice, and the ratio of docosapentaenoic acid (DPA, 22:5n-3) to DHA in P1 KO liver was higher, suggesting lower desaturase activity. Concomitantly, changes in other fatty acid levels and related gene expressions were observed, indicating that Fads3 may enhance liver-mediated 22:6n-3 synthesis to support brain 22:6n-3 accretion before and during the brain growth spurt [5].
In conclusion, Fads3 has diverse functions in lipid metabolism, playing a role in sphingolipid biosynthesis and influencing fatty acid composition in the liver and brain. The Fads3 KO mouse model has provided insights into its role in supporting brain DHA accretion, and further research on Fads3 may offer a better understanding of lipid-related biological processes and potentially related disease mechanisms [5].
References:
1. Rioux, Vincent, Legrand, Philippe. 2019. Fatty Acid Desaturase 3 (FADS3) Is a Specific ∆13-Desaturase of Ruminant trans-Vaccenic Acid. In Lifestyle genomics, 12, 18-24. doi:10.1159/000502356. https://pubmed.ncbi.nlm.nih.gov/32911476/
2. Jojima, Keisuke, Kihara, Akio. 2023. Metabolism of sphingadiene and characterization of the sphingadiene-producing enzyme FADS3. In Biochimica et biophysica acta. Molecular and cell biology of lipids, 1868, 159335. doi:10.1016/j.bbalip.2023.159335. https://pubmed.ncbi.nlm.nih.gov/37209771/
3. Karsai, Gergely, Lone, Museer, Kutalik, Zoltán, von Eckardstein, Arnold, Hornemann, Thorsten. 2019. FADS3 is a Δ14Z sphingoid base desaturase that contributes to gender differences in the human plasma sphingolipidome. In The Journal of biological chemistry, 295, 1889-1897. doi:10.1074/jbc.AC119.011883. https://pubmed.ncbi.nlm.nih.gov/31862735/
4. Blanchard, H, Legrand, P, Pédrono, F. 2010. Fatty Acid Desaturase 3 (Fads3) is a singular member of the Fads cluster. In Biochimie, 93, 87-90. doi:10.1016/j.biochi.2010.03.002. https://pubmed.ncbi.nlm.nih.gov/20226833/
5. Zhang, Ji Yao, Qin, Xia, Liang, Allison, Kothapalli, Kumar S D, Brenna, J Thomas. 2017. Fads3 modulates docosahexaenoic acid in liver and brain. In Prostaglandins, leukotrienes, and essential fatty acids, 123, 25-32. doi:10.1016/j.plefa.2017.07.001. https://pubmed.ncbi.nlm.nih.gov/28838557/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen