C57BL/6JCya-Gpr52em1/Cya
Common Name
Gpr52-KO
Product ID
S-KO-11241
Backgroud
C57BL/6JCya
Strain ID
KOCMP-620246-Gpr52-B6J-VA
Status
When using this mouse strain in a publication, please cite “Gpr52-KO Mouse (Catalog S-KO-11241) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Gpr52-KO
Strain ID
KOCMP-620246-Gpr52-B6J-VA
Gene Name
Product ID
S-KO-11241
Gene Alias
EG620246
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000238289
NCBI RefSeq
NM_001146330
Target Region
Exon 1
Size of Effective Region
~1.6 kb
Overview of Gene Research
Gpr52 is an orphan G protein-coupled receptor (GPCR) that is highly conserved and enriched in the brain. It is a Gs/olf-coupled receptor controlling various cyclic AMP (cAMP)-dependent physiological and pathological processes [1].
In a study using HdhQ140 mice, striatal knockdown of Gpr52 reduced mHTT levels, validating Gpr52 as a potential target for Huntington's disease (HD) [2]. In breast cancer research, knockout of Gpr52 in human triple-negative breast cancer (TNBC) cell lines and non-cancerous breast epithelial cell line MCF10A using CRISPR-Cas9 was associated with increased cell-cell interaction, altered 3D spheroid morphology, and increased invasion, suggesting a role in breast cancer progression [3].
In conclusion, Gpr52 plays a crucial role in cAMP-dependent physiological and pathological processes. The gene knockout models in mice and cell lines have revealed its potential as a therapeutic target in diseases such as Huntington's disease and breast cancer, providing insights into its function in these disease conditions.
References:
1. Ali, Saghir, Wang, Pingyuan, Murphy, Ryan E, Allen, John A, Zhou, Jia. 2024. Orphan GPR52 as an emerging neurotherapeutic target. In Drug discovery today, 29, 103922. doi:10.1016/j.drudis.2024.103922. https://pubmed.ncbi.nlm.nih.gov/38387741/
2. Wang, Congcong, Zhang, Yu-Fang, Guo, Shimeng, Lu, Boxun, Hu, Youhong. 2020. GPR52 Antagonist Reduces Huntingtin Levels and Ameliorates Huntington's Disease-Related Phenotypes. In Journal of medicinal chemistry, 64, 941-957. doi:10.1021/acs.jmedchem.0c01133. https://pubmed.ncbi.nlm.nih.gov/33185430/
3. Hanif, Sarah Z, Au, CheukMan Cherie, Torregroza, Ingrid, Hébert, Terence E, Brown, Kristy A. 2024. The Orphan G Protein-Coupled Receptor GPR52 is a Novel Regulator of Breast Cancer Multicellular Organization. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.07.22.604482. https://pubmed.ncbi.nlm.nih.gov/39091857/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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