C57BL/6JCya-Gpr35em1/Cya
Common Name
Gpr35-KO
Product ID
S-KO-11392
Backgroud
C57BL/6JCya
Strain ID
KOCMP-64095-Gpr35-B6J-VA
Status
When using this mouse strain in a publication, please cite “Gpr35-KO Mouse (Catalog S-KO-11392) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Gpr35-KO
Strain ID
KOCMP-64095-Gpr35-B6J-VA
Gene Name
Product ID
S-KO-11392
Gene Alias
KPG_007
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000169198
NCBI RefSeq
NM_022320
Target Region
Exon 2
Size of Effective Region
~2.6 kb
Overview of Gene Research
Gpr35, a G protein-coupled receptor, plays a notable role in modulating inflammatory responses [1]. It has been associated with multiple biological pathways, such as the gut-microbe-brain metabolic axis and neutrophil recruitment. Gpr35 can be activated by various ligands including kynurenic acid, 5-hydroxyindoleacetic acid (5-HIAA), and others. The use of genetic models, like gene knockout (KO) mouse models, has been crucial in understanding its function [4,5].
In KO mouse models, genetic deletion of Gpr35 in the gut epithelium induces depressive-like behavior in a microbiome-dependent manner, suggesting its role in the gut-microbe-brain axis regulating depression [2]. Also, Gpr35-deficient neutrophils are less recruited from blood vessels into inflamed tissue, and the mice are less efficient in clearing peritoneal bacteria, highlighting its importance in innate immune responses [3].
In conclusion, Gpr35 is essential in regulating inflammation, gut-microbe-brain-related behaviors, and neutrophil-mediated innate immune responses. The gene knockout mouse models have significantly contributed to understanding its role in depression-related behaviors and immune-mediated bacterial clearance, providing insights into potential therapeutic targets for inflammatory diseases and mental health disorders.
References:
1. Wu, Yetian, Zhang, Pei, Fan, Hongjie, Liang, Xinmiao, Chen, Yang. 2023. GPR35 acts a dual role and therapeutic target in inflammation. In Frontiers in immunology, 14, 1254446. doi:10.3389/fimmu.2023.1254446. https://pubmed.ncbi.nlm.nih.gov/38035084/
2. Cheng, Lingsha, Wu, Haoqian, Cai, Xiaoying, Hao, Haiping, Zheng, Xiao. 2024. A Gpr35-tuned gut microbe-brain metabolic axis regulates depressive-like behavior. In Cell host & microbe, 32, 227-243.e6. doi:10.1016/j.chom.2023.12.009. https://pubmed.ncbi.nlm.nih.gov/38198925/
3. De Giovanni, Marco, Tam, Hanson, Valet, Colin, Looney, Mark R, Cyster, Jason G. 2022. GPR35 promotes neutrophil recruitment in response to serotonin metabolite 5-HIAA. In Cell, 185, 815-830.e19. doi:10.1016/j.cell.2022.01.010. https://pubmed.ncbi.nlm.nih.gov/35148838/
4. Kaya, Berna, Melhem, Hassan, Niess, Jan Hendrik. 2021. GPR35 in Intestinal Diseases: From Risk Gene to Function. In Frontiers in immunology, 12, 717392. doi:10.3389/fimmu.2021.717392. https://pubmed.ncbi.nlm.nih.gov/34790192/
5. Im, Dong-Soon. 2023. Recent advances in GPR35 pharmacology; 5-HIAA serotonin metabolite becomes a ligand. In Archives of pharmacal research, 46, 550-563. doi:10.1007/s12272-023-01449-y. https://pubmed.ncbi.nlm.nih.gov/37227682/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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