C57BL/6JCya-Pmepa1em1/Cya
Common Name
Pmepa1-KO
Product ID
S-KO-11467
Backgroud
C57BL/6JCya
Strain ID
KOCMP-65112-Pmepa1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Pmepa1-KO Mouse (Catalog S-KO-11467) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Pmepa1-KO
Strain ID
KOCMP-65112-Pmepa1-B6J-VA
Gene Name
Product ID
S-KO-11467
Gene Alias
Stag1, Erg1.2, N4wbp4, Tmepai, 2210418I02Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000036248
NCBI RefSeq
NM_022995
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Overview of Gene Research
Pmepa1, also known as prostate transmembrane protein androgen induced 1, is an androgen-responsive gene. It serves as an essential regulator of multiple signaling pathways, such as androgen and TGF-β signaling in solid tumors. PMEPA1 has five isoforms with distinct extracellular domains, and specific motifs in its cytoplasmic domain mediate isoform-specific functions through protein-protein interactions [1].
In hepatocellular carcinoma (HCC), overexpression of MYC + PMEPA1 in a genetically engineered mouse model led to HCC development in about 60% of mice, with decreased survival compared to mice overexpressing MYC alone. This demonstrated the oncogenic role of PMEPA1 in HCC, as it was linked to TGF-β activation, immune exhaustion, and an aggressive phenotype [2]. In osteomyelitis, knockdown of Pmepa1 in a rat model alleviated SpA-induced pyroptosis and osteogenic differentiation inhibition of human bone marrow mesenchymal stem cells (hBMSCs) by downregulating the p38MAPK/NLRP3 signaling axis, suggesting that modulating Pmepa1 expression may be a potential strategy for treating osteomyelitis [3].
In conclusion, Pmepa1 is a key regulator of multiple signaling pathways. Its study using mouse models has revealed its oncogenic role in HCC and its potential as a therapeutic target in osteomyelitis. These findings enhance our understanding of disease pathogenesis and may open new avenues for targeted therapeutic interventions.
References:
1. Sharad, Shashwat, Dobi, Albert, Srivastava, Shiv, Srinivasan, Alagarsamy, Li, Hua. 2020. PMEPA1 Gene Isoforms: A Potential Biomarker and Therapeutic Target in Prostate Cancer. In Biomolecules, 10, . doi:10.3390/biom10091221. https://pubmed.ncbi.nlm.nih.gov/32842649/
2. Piqué-Gili, Marta, Andreu-Oller, Carmen, Mesropian, Agavni, Pinyol, Roser, Llovet, Josep M. 2024. Oncogenic role of PMEPA1 and its association with immune exhaustion and TGF-β activation in HCC. In JHEP reports : innovation in hepatology, 6, 101212. doi:10.1016/j.jhepr.2024.101212. https://pubmed.ncbi.nlm.nih.gov/39524206/
3. Li, Mingjun, Shi, Xiangwen, Wu, Yipeng, Zhang, Bihuan, Xu, Yongqing. 2023. Pmepa1 knockdown alleviates SpA-induced pyroptosis and osteogenic differentiation inhibition of hBMSCs via p38MAPK/NLRP3 axis. In International immunopharmacology, 124, 110843. doi:10.1016/j.intimp.2023.110843. https://pubmed.ncbi.nlm.nih.gov/37634444/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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