C57BL/6JCya-Fmc1em1/Cya
Common Name:
Fmc1-KO
Product ID:
S-KO-11541
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fmc1-KO
Strain ID
KOCMP-66117-Fmc1-B6J-VA
Gene Name
Product ID
S-KO-11541
Gene Alias
1110001J03Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fmc1em1/Cya mice (Catalog S-KO-11541) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000019833
NCBI RefSeq
NM_025363
Target Region
Exon 1~2
Size of Effective Region
~4.5 kb
Detailed Document
Overview of Gene Research
Fmc1, a nuclear gene in yeast, is crucial for the assembly/stability of the mitochondrial F₁ sector of the ATP synthase at elevated temperatures (37 °C). It codes for a soluble protein located in the mitochondrial matrix and is associated with the oxidative phosphorylation pathway, which is vital for energy production in cells [1].
In yeast cells lacking Fmc1p, at 37 °C, the α-F₁ and β-F₁ proteins are synthesized, transported, and processed but form aggregates instead of incorporating into a functional F₁ oligomer. However, increasing the expression of Atp12p can compensate for the absence of Fmc1p. The deletion mutant Δfmc1 shows very slow growth on respiratory substrates at 37 °C, which can be rescued by overexpressing Odc1p. This rescuing mechanism involves increasing the flux of tricarboxylic acid cycle intermediates into mitochondria, leading to more substrate-level-dependent ATP synthesis [1,2].
In conclusion, Fmc1 is essential for proper mitochondrial F₁-ATPase assembly and stability under heat stress conditions in yeast. The study of Fmc1 in yeast models provides insights into the molecular mechanisms underlying mitochondrial function and energy metabolism, which may have implications for understanding related pathologies associated with oxidative phosphorylation system defects in higher organisms [1,2].
References:
1. Lefebvre-Legendre, L, Vaillier, J, Benabdelhak, H, Slonimski, P P, di Rago, J P. 2000. Identification of a nuclear gene (FMC1) required for the assembly/stability of yeast mitochondrial F(1)-ATPase in heat stress conditions. In The Journal of biological chemistry, 276, 6789-96. doi:. https://pubmed.ncbi.nlm.nih.gov/11096112/
2. Schwimmer, Christine, Lefebvre-Legendre, Linnka, Rak, Malgorzata, di Rago, Jean-Paul, Rigoulet, Michel. 2005. Increasing mitochondrial substrate-level phosphorylation can rescue respiratory growth of an ATP synthase-deficient yeast. In The Journal of biological chemistry, 280, 30751-9. doi:. https://pubmed.ncbi.nlm.nih.gov/15975925/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen