C57BL/6NCya-Zfp541em1/Cya
Common Name:
Zfp541-KO
Product ID:
S-KO-11836
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Zfp541-KO
Strain ID
KOCMP-666528-Zfp541-B6N-VA
Gene Name
Product ID
S-KO-11836
Gene Alias
EG666528; Znf541
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Zfp541em1/Cya mice (Catalog S-KO-11836) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000108509
NCBI RefSeq
NM_001099277
Target Region
Exon 2~3
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Zfp541, also known as SHIP1 (spermatogenic cell HDAC-interacting protein 1), is a germ-cell-specific gene essential for male meiosis and fertility [5]. It is involved in pathways related to chromatin organization and transcriptional regulation during spermatogenesis [1,2,4]. Mouse models are crucial for studying its function as they can mimic the in-vivo situation to understand its role in biological processes.
In Zfp541 knockout male mice, various defects occur. There are structural and recombination defects such as detached chromosome ends, abnormal localization of chromosome axis components and recombination proteins, and globally altered histone modifications [1]. Zfp541 -/- spermatocytes show abnormal programmed DNA double-strand break repair, impaired crossover formation and resolution, and asynapsis of the XY chromosomes, leading to meiotic arrest at the diplotene stage [2,3]. Additionally, Zfp541 deletion causes aberrant recruitment of DSB machinery to chromosome axes and generation of massive DSBs in late pachytene and diplotene spermatocytes [3].
In conclusion, Zfp541 is essential for male meiosis, acting as a key transcriptional regulator. It simultaneously regulates the transcription cascade and chromatin organization to ensure the coordinated progression of meiosis prophase I. The Zfp541 knockout mouse models have significantly contributed to understanding male infertility, as Zfp541 deficiency leads to various meiotic defects that can cause infertility [1-5].
References:
1. Zhou, Xu, Fang, Kailun, Liu, Yanlei, Chen, Charlie Degui, Wang, Shunxin. 2023. ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression. In Cell proliferation, 57, e13567. doi:10.1111/cpr.13567. https://pubmed.ncbi.nlm.nih.gov/37921559/
2. Li, Yushan, Meng, Ranran, Li, Shanze, Xu, Dan, Wang, Fengchao. 2022. The ZFP541-KCTD19 complex is essential for pachytene progression by activating meiotic genes during mouse spermatogenesis. In Journal of genetics and genomics = Yi chuan xue bao, 49, 1029-1041. doi:10.1016/j.jgg.2022.03.005. https://pubmed.ncbi.nlm.nih.gov/35341968/
3. Xu, Jianze, Gao, Jianing, Liu, Junyan, Ma, Hui, Shi, Qinghua. . ZFP541 maintains the repression of pre-pachytene transcriptional programs and promotes male meiosis progression. In Cell reports, 38, 110540. doi:10.1016/j.celrep.2022.110540. https://pubmed.ncbi.nlm.nih.gov/35320728/
4. Horisawa-Takada, Yuki, Kodera, Chisato, Takemoto, Kazumasa, Araki, Kimi, Ishiguro, Kei-Ichiro. 2021. Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis. In Nature communications, 12, 3184. doi:10.1038/s41467-021-23378-4. https://pubmed.ncbi.nlm.nih.gov/34075040/
5. Choi, Eunyoung, Han, Cecil, Park, Inju, Eddy, Edward M, Cho, Chunghee. 2008. A novel germ cell-specific protein, SHIP1, forms a complex with chromatin remodeling activity during spermatogenesis. In The Journal of biological chemistry, 283, 35283-94. doi:10.1074/jbc.M805590200. https://pubmed.ncbi.nlm.nih.gov/18849567/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen