C57BL/6NCya-Piezo2em1/Cya
Common Name:
Piezo2-KO
Product ID:
S-KO-11907
Background:
C57BL/6NCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Piezo2-KO
Strain ID
KOCMP-667742-Piezo2-B6N-VA
Gene Name
Product ID
S-KO-11907
Gene Alias
5930434P17; 9030411M15Rik; 9430028L06Rik; Fam38b; Fam38b2
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Piezo2em1/Cya mice (Catalog S-KO-11907) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000047480
NCBI RefSeq
NM_001039485
Target Region
Exon 6~7
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Piezo2, also known as Fam38B, is a vertebrate multipass transmembrane protein and an essential component of mechanically activated cation channels [1]. Mechanosensation, which Piezo2 is involved in, is crucial for numerous physiological processes such as touch, proprioception, and bladder stretch sensation [2,3].
In KO mouse models, Piezo2-deficient proprioceptive neurons led to severely uncoordinated body movements and abnormal limb positions, indicating that Piezo2 is the major mechanotransducer of mammalian proprioceptors [4]. In Merkel-cell-specific Piezo2-deficient mice, Merkel-cell mechanosensitivity was completely lost, and in vivo slowly adapting responses mediated by the Merkel cell-neurite complex had reduced static firing rates [7]. Nociceptor-specific Piezo2 conditional knockout mice were protected from mechanical sensitization associated with inflammatory joint pain and osteoarthritis [6]. In humans, loss-of-function mutations in PIEZO2 result in an autosomal recessive syndrome of muscular atrophy with perinatal respiratory distress, arthrogryposis, and scoliosis [5].
In conclusion, Piezo2 is vital for mechanosensation in various physiological processes. Studies using KO and CKO mouse models have revealed its role in proprioception, Merkel-cell mechanotransduction, and mechanical sensitization in diseases like osteoarthritis. Understanding Piezo2 through these research models provides insights into the molecular mechanisms of mechanosensation and potential therapeutic targets for related diseases.
References:
1. Coste, Bertrand, Mathur, Jayanti, Schmidt, Manuela, Dubin, Adrienne E, Patapoutian, Ardem. 2010. Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels. In Science (New York, N.Y.), 330, 55-60. doi:10.1126/science.1193270. https://pubmed.ncbi.nlm.nih.gov/20813920/
2. Szczot, Marcin, Nickolls, Alec R, Lam, Ruby M, Chesler, Alexander T. . The Form and Function of PIEZO2. In Annual review of biochemistry, 90, 507-534. doi:10.1146/annurev-biochem-081720-023244. https://pubmed.ncbi.nlm.nih.gov/34153212/
3. Villarino, Nicholas W, Hamed, Yasmeen M F, Ghosh, Britya, Patapoutian, Ardem, Marshall, Kara L. 2023. Labeling PIEZO2 activity in the peripheral nervous system. In Neuron, 111, 2488-2501.e8. doi:10.1016/j.neuron.2023.05.015. https://pubmed.ncbi.nlm.nih.gov/37321223/
4. Woo, Seung-Hyun, Lukacs, Viktor, de Nooij, Joriene C, Wilkinson, Katherine A, Patapoutian, Ardem. 2015. Piezo2 is the principal mechanotransduction channel for proprioception. In Nature neuroscience, 18, 1756-62. doi:10.1038/nn.4162. https://pubmed.ncbi.nlm.nih.gov/26551544/
5. Alper, S L. 2017. Genetic Diseases of PIEZO1 and PIEZO2 Dysfunction. In Current topics in membranes, 79, 97-134. doi:10.1016/bs.ctm.2017.01.001. https://pubmed.ncbi.nlm.nih.gov/28728825/
6. Obeidat, Alia M, Wood, Matthew J, Adamczyk, Natalie S, Malfait, Anne-Marie, Miller, Rachel E. 2023. Piezo2 expressing nociceptors mediate mechanical sensitization in experimental osteoarthritis. In Nature communications, 14, 2479. doi:10.1038/s41467-023-38241-x. https://pubmed.ncbi.nlm.nih.gov/37120427/
7. Woo, Seung-Hyun, Ranade, Sanjeev, Weyer, Andy D, Stucky, Cheryl L, Patapoutian, Ardem. 2014. Piezo2 is required for Merkel-cell mechanotransduction. In Nature, 509, 622-6. doi:10.1038/nature13251. https://pubmed.ncbi.nlm.nih.gov/24717433/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen