C57BL/6JCya-Resf1em1/Cya
Common Name
Resf1-KO
Product ID
S-KO-12186
Backgroud
C57BL/6JCya
Strain ID
KOCMP-67246-Resf1-B6J-VA
When using this mouse strain in a publication, please cite “Resf1-KO Mouse (Catalog S-KO-12186) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Resf1-KO
Strain ID
KOCMP-67246-Resf1-B6J-VA
Gene Name
Product ID
S-KO-12186
Gene Alias
2810474O19Rik, 6720435I21Rik, GET
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000046689
NCBI RefSeq
NM_026054
Target Region
Exon 3~5
Size of Effective Region
~10.3 kb
Overview of Gene Research
Resf1, also known as Retroelement silencing factor 1, has multiple important functions. It is involved in transcriptional control, retroelement silencing, and plays a role in the regulation of pluripotent stem cells and germ cell specification [1,2,3]. Genetic models like KO mouse models are valuable for studying its functions.
In triple negative breast cancer, CRISPR or gene trap mouse models crossed to MMTV-PyMT showed that reduction of Resf1 led to increased tumor growth, shortened survival, and more lung metastases, indicating it is a tumor suppressor. Mechanistically, it may be involved in transcriptional control via association with compound G4 quadruplexes [1]. In embryonic stem cells, Resf1 knockout reduced self-renewal potential and the efficiency of differentiation into primordial germ cell-like cells [2]. A CRISPR knockout screen in mouse embryonic stem cells identified Resf1 as a novel molecule in retroelement silencing, where it regulates repressive epigenetic modifications associated with SETDB1 [3].
In conclusion, Resf1 is crucial for maintaining normal cell function in processes such as stem cell self-renewal, germ cell specification, and retroelement silencing. Its loss-of-function in KO mouse models reveals its tumor-suppressing role in triple negative breast cancer, highlighting its significance in understanding cancer biology and potentially developing new therapeutic strategies [1,2,3].
References:
1. Majocha, Megan R, Jackson, Devin E, Ha, Ngoc-Han, Lee, Maxwell P, Hunter, Kent W. 2024. Resf1 is a compound G4 quadruplex-associated tumor suppressor for triple negative breast cancer. In PLoS genetics, 20, e1011236. doi:10.1371/journal.pgen.1011236. https://pubmed.ncbi.nlm.nih.gov/38722825/
2. Vojtek, Matúš, Chambers, Ian. 2021. Loss of Resf1 reduces the efficiency of embryonic stem cell self-renewal and germline entry. In Life science alliance, 4, . doi:10.26508/lsa.202101190. https://pubmed.ncbi.nlm.nih.gov/34607919/
3. Fukuda, Kei, Okuda, Akihiko, Yusa, Kosuke, Shinkai, Yoichi. 2018. A CRISPR knockout screen identifies SETDB1-target retroelement silencing factors in embryonic stem cells. In Genome research, 28, 846-858. doi:10.1101/gr.227280.117. https://pubmed.ncbi.nlm.nih.gov/29728365/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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