C57BL/6JCya-Tm7sf3em1/Cya
Common Name
Tm7sf3-KO
Product ID
S-KO-12370
Backgroud
C57BL/6JCya
Strain ID
KOCMP-67623-Tm7sf3-B6J-VA
When using this mouse strain in a publication, please cite “Tm7sf3-KO Mouse (Catalog S-KO-12370) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tm7sf3-KO
Strain ID
KOCMP-67623-Tm7sf3-B6J-VA
Gene Name
Product ID
S-KO-12370
Gene Alias
2010003B14Rik, NARP1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000037709
NCBI RefSeq
NM_026281
Target Region
Exon 2~5
Size of Effective Region
~6.4 kb
Overview of Gene Research
Tm7sf3, or transmembrane 7 superfamily member 3, is a nuclear seven-transmembrane protein with multiple important functions. It is a p53-regulated homeostatic factor that can attenuate cellular stress and the unfolded protein response [3]. It localizes to nuclear speckles and is involved in regulating alternative splicing of numerous genes, mainly at the 3' end of introns by modulating splicing factors like HNRNPK [2].
Deletion of Tm7sf3 in liver organoids, primary human hepatic stellate cells (HSCs), and in vivo in metabolic-dysfunction-associated steatohepatitis (MASH) mice accelerates HSC activation, leading to the activation of the fibrogenic program and HSC proliferation. This occurs because Tm7sf3 knockdown promotes alternative splicing of the Hippo pathway transcription factor TEAD1 by inhibiting the splicing factor hnRNPU, generating a more active form of TEAD1 and triggering HSC activation [1].
In conclusion, Tm7sf3 is crucial for maintaining cellular homeostasis and regulating alternative splicing. Its deletion in MASH mouse models reveals its key role in preventing HSC activation and the progression of MASH-related fibrosis, highlighting its potential as a therapeutic target for liver fibrosis.
References:
1. Isaac, Roi, Bandyopadhyay, Gautam, Rohm, Theresa V, Webster, Nicholas J G, Olefsky, Jerrold M. 2024. TM7SF3 controls TEAD1 splicing to prevent MASH-induced liver fibrosis. In Cell metabolism, 36, 1030-1043.e7. doi:10.1016/j.cmet.2024.04.003. https://pubmed.ncbi.nlm.nih.gov/38670107/
2. Isaac, Roi, Vinik, Yaron, Mikl, Martin, Elhanany, Eytan, Zick, Yehiel. 2022. A seven-transmembrane protein-TM7SF3, resides in nuclear speckles and regulates alternative splicing. In iScience, 25, 105270. doi:10.1016/j.isci.2022.105270. https://pubmed.ncbi.nlm.nih.gov/36304109/
3. Isaac, Roi, Goldstein, Ido, Furth, Noa, Oren, Moshe, Zick, Yehiel. 2016. TM7SF3, a novel p53-regulated homeostatic factor, attenuates cellular stress and the subsequent induction of the unfolded protein response. In Cell death and differentiation, 24, 132-143. doi:10.1038/cdd.2016.108. https://pubmed.ncbi.nlm.nih.gov/27740623/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.