C57BL/6NCya-Armc12em1/Cya
Common Name
Armc12-KO
Product ID
S-KO-12375
Backgroud
C57BL/6NCya
Strain ID
KOCMP-67645-Armc12-B6N-VA
Status
When using this mouse strain in a publication, please cite “Armc12-KO Mouse (Catalog S-KO-12375) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Armc12-KO
Strain ID
KOCMP-67645-Armc12-B6N-VA
Gene Name
Product ID
S-KO-12375
Gene Alias
4930511I11Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 17
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000233923
NCBI RefSeq
NM_026290
Target Region
Exon 1~6
Size of Effective Region
~8.0 kb
Overview of Gene Research
Armc12, an armadillo repeat-containing protein, is a mitochondrial peripheral membrane protein. It plays a crucial role in regulating mitochondrial dynamics, especially during spermiogenesis. It is involved in the formation of the mitochondrial sheath in sperm, which is essential for sperm motility, thus being of great importance for male fertility [1,2]. Genetic models like knockout mice are valuable tools for studying its functions.
In Armc12-null mice, abnormal mitochondrial coiling along the flagellum occurs due to improper elongation at the mitochondrial interlocking step during spermiogenesis. This leads to reduced sperm motility and male sterility. ARMC12 functions as an adherence factor between mitochondria in cultured cells. It interacts with mitochondrial proteins such as MIC60, VDAC2, VDAC3, as well as TBC1D21 and GK2 in testicular germ cells, with TBC1D21 being essential for the interaction between ARMC12 and VDAC proteins in vivo. These interactions are required for mitochondrial sheath formation [1]. In human studies, biallelic mutations in ARMC12 were identified in patients with asthenozoospermia, whose spermatozoa showed multiple midpiece defects similar to those in Armc12-knockout mice [2].
In conclusion, Armc12 is essential for regulating spatiotemporal mitochondrial dynamics to form the mitochondrial sheath during spermiogenesis. The study of Armc12-null mouse models has significantly contributed to understanding male infertility caused by asthenozoospermia and multiple midpiece defects in humans [1,2].
References:
1. Shimada, Keisuke, Park, Soojin, Miyata, Haruhiko, Matzuk, Martin M, Ikawa, Masahito. . ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. In Proceedings of the National Academy of Sciences of the United States of America, 118, . doi:10.1073/pnas.2018355118. https://pubmed.ncbi.nlm.nih.gov/33536340/
2. Liu, Wensheng, Wei, Xiaoli, Liu, Xiaoyan, Sha, Yanwei, Wang, Yifeng. 2022. Biallelic mutations in ARMC12 cause asthenozoospermia and multiple midpiece defects in humans and mice. In Journal of medical genetics, 60, 154-162. doi:10.1136/jmedgenet-2021-108137. https://pubmed.ncbi.nlm.nih.gov/35534203/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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