C57BL/6JCya-Alkbh8em1/Cya
Common Name
Alkbh8-KO
Product ID
S-KO-12386
Backgroud
C57BL/6JCya
Strain ID
KOCMP-67667-Alkbh8-B6J-VA
Status
When using this mouse strain in a publication, please cite “Alkbh8-KO Mouse (Catalog S-KO-12386) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Alkbh8-KO
Strain ID
KOCMP-67667-Alkbh8-B6J-VA
Gene Name
Product ID
S-KO-12386
Gene Alias
Abh8, 4930562C03Rik, 8030431D03Rik, 9430088N01Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 9
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000165105
NCBI RefSeq
NM_026303
Target Region
Exon 2~3
Size of Effective Region
~2.5 kb
Overview of Gene Research
Alkbh8, or AlkB homolog 8, is a methyltransferase involved in tRNA modification. It methylates the anticodon wobble uridine residue of tRNAs, regulating selenoprotein translation [2]. This process is essential for proper protein translation and is linked to several biological functions and disease pathophysiologies [1].
Alkbh8-knockout (Albkh8 -/-) mice studies have revealed its significance. These mice showed reduced 5-methoxycarbonylmethyluridine levels, abnormal behaviors indicating central nervous system dysfunction, and ischemic pathological changes in the cerebral cortex and hippocampus [1]. Neurons and glial cells from these mice also had reduced mitochondrial membrane potential [1]. In Drosophila, ALKBH8 null animals lacked wobble uridine methylation, had reduced protein synthesis in the nervous system, and exhibited ectopic synapse formation and memory impairments, which were reversed by antioxidant treatment [3].
In conclusion, Alkbh8 is crucial for maintaining neural function through oxidative stress-regulatory mechanisms and for proper synapse formation and memory in the nervous system. Its loss-of-function models have provided insights into its role in central nervous system-related diseases and intellectual disability disorders [1,3].
References:
1. Honda, Kohei, Hase, Hiroaki, Tanikawa, Sayaka, Nakagawa, Shinsaku, Tsujikawa, Kazutake. 2024. ALKBH8 contributes to neurological function through oxidative stress regulation. In PNAS nexus, 3, pgae115. doi:10.1093/pnasnexus/pgae115. https://pubmed.ncbi.nlm.nih.gov/38550277/
2. Maddirevula, Sateesh, Alameer, Seham, Ewida, Nour, Vågbø, Cathrine Broberg, Alkuraya, Fowzan S. 2021. Insight into ALKBH8-related intellectual developmental disability based on the first pathogenic missense variant. In Human genetics, 141, 209-215. doi:10.1007/s00439-021-02391-z. https://pubmed.ncbi.nlm.nih.gov/34757492/
3. Madhwani, Kimberly R, Sayied, Shanzeh, Ogata, Carlson H, Fu, Dragony, O'Connor-Giles, Kate M. 2024. tRNA modification enzyme-dependent redox homeostasis regulates synapse formation and memory. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2317864121. doi:10.1073/pnas.2317864121. https://pubmed.ncbi.nlm.nih.gov/39495910/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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