Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Naa20em1/Cya
Common Name:
Naa20-KO
Product ID:
S-KO-12494
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Naa20-KO
Strain ID
KOCMP-67877-Naa20-B6J-VA
Gene Name
Naa20
Product ID
S-KO-12494
Gene Alias
1500004D14Rik; 2900026I01Rik; D2Ertd186e; Nat5
Background
C57BL/6JCya
NCBI ID
67877
Modification
Conventional knockout
Chromosome
2
Phenotype
MGI:1915127
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Naa20em1/Cya mice (Catalog S-KO-12494) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000002805
NCBI RefSeq
NM_026425
Target Region
Exon 2~3
Size of Effective Region
~3.2 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Naa20, also known as N-α-acetyltransferase 20, is the catalytic subunit of the NatB complex. NatB is responsible for N-terminal acetylation of approximately 20% of the human proteome, a modification crucial for protein homeostasis, localization, and interactions [2,3,4]. This process is linked to various biological processes and human diseases [3]. Genetic models, such as in yeast and mice, have been valuable for studying Naa20's functions [6,7].

In triple-negative breast cancer (TNBC), Naa20 expression is higher in cancer tissues. Knockdown of Naa20 in TNBC cells inhibits cell viability, migration, and invasion, and reduces EGFR expression. Naa20 interacts with Rab5A, and over-expression of Naa20 potentiates Rab5A expression. Knockdown of Rab5A inhibits EGFR and downstream signaling, while Naa20 over-expression offsets this effect. In vivo, Naa20 knockdown suppresses tumor growth in a xenograft mouse model [1]. In hepatocellular carcinoma (HCC), Naa20 promotes oncogenic properties by inhibiting the LKB1-AMPK-mTOR axis [5]. In NatB-deficient mouse embryonic fibroblasts (Naa20 -/-), there is reduced responsiveness to an extrinsic pro-apoptotic stimulus due to procaspase-8 degradation [7].

In conclusion, Naa20, as a key component of the NatB complex, plays significant roles in protein N-terminal acetylation, which impacts multiple biological processes. Model-based research, especially the use of KO mouse models, has revealed its importance in diseases like TNBC and HCC, highlighting its potential as a therapeutic target.

References:

1. Qiao, Lei, Dong, Chao, Jia, Wenlei, Ma, Binlin. 2023. NAA20 recruits Rin2 and promotes triple-negative breast cancer progression by regulating Rab5A-mediated activation of EGFR signaling. In Cellular signalling, 112, 110922. doi:10.1016/j.cellsig.2023.110922. https://pubmed.ncbi.nlm.nih.gov/37827343/

2. D'Onofrio, Gianluca, Cuccurullo, Claudia, Larsen, Silje Kathrine, Arnesen, Thomas, Bilo, Leonilda. 2023. Novel biallelic variants expand the phenotype of NAA20-related syndrome. In Clinical genetics, 104, 371-376. doi:10.1111/cge.14359. https://pubmed.ncbi.nlm.nih.gov/37191084/

3. Layer, Dominik, Kopp, Jürgen, Fontanillo, Miriam, Lapouge, Karine, Sinning, Irmgard. 2021. Structural basis of Naa20 activity towards a canonical NatB substrate. In Communications biology, 4, 2. doi:10.1038/s42003-020-01546-4. https://pubmed.ncbi.nlm.nih.gov/33398031/

4. Morrison, Jennifer, Altuwaijri, Norah K, Brønstad, Kirsten, Alkuraya, Fowzan S, Arnesen, Thomas. 2021. Missense NAA20 variants impairing the NatB protein N-terminal acetyltransferase cause autosomal recessive developmental delay, intellectual disability, and microcephaly. In Genetics in medicine : official journal of the American College of Medical Genetics, 23, 2213-2218. doi:10.1038/s41436-021-01264-0. https://pubmed.ncbi.nlm.nih.gov/34230638/

5. Jung, Taek-Yeol, Ryu, Jae-Eun, Jang, Mi-Mi, Ju, Bong-Gun, Kim, Hyun-Seok. 2020. Naa20, the catalytic subunit of NatB complex, contributes to hepatocellular carcinoma by regulating the LKB1-AMPK-mTOR axis. In Experimental & molecular medicine, 52, 1831-1844. doi:10.1038/s12276-020-00525-3. https://pubmed.ncbi.nlm.nih.gov/33219302/

6. Lee, Kang-Eun, Ahn, Jun-Young, Kim, Jeong-Mok, Hwang, Cheol-Sang. 2014. Synthetic lethal screen of NAA20, a catalytic subunit gene of NatB N-terminal acetylase in Saccharomyces cerevisiae. In Journal of microbiology (Seoul, Korea), 52, 842-8. doi:10.1007/s12275-014-3694-z. https://pubmed.ncbi.nlm.nih.gov/25163837/

7. Guedes, Joana P, Boyer, Jean Baptiste, Elurbide, Jasmine, Giglione, Carmela, Aldabe, Rafael. 2024. NatB Protects Procaspase-8 from UBR4-Mediated Degradation and Is Required for Full Induction of the Extrinsic Apoptosis Pathway. In Molecular and cellular biology, 44, 358-371. doi:10.1080/10985549.2024.2382453. https://pubmed.ncbi.nlm.nih.gov/39099191/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest