C57BL/6JCya-Ucmaem1/Cya
Common Name:
Ucma-KO
Product ID:
S-KO-12746
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ucma-KO
Strain ID
KOCMP-68527-Ucma-B6J-VA
Gene Name
Product ID
S-KO-12746
Gene Alias
1110017I16Rik; Grp
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ucmaem1/Cya mice (Catalog S-KO-12746) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000115010
NCBI RefSeq
NM_001113558
Target Region
Exon 1~5
Size of Effective Region
~9.3 kb
Detailed Document
Overview of Gene Research
Ucma, also known as Upper zone of growth plate and Cartilage Matrix Associated protein or Gla Rich Protein (GRP), is a highly conserved tyrosine-sulphated secreted protein. It is a member of the vitamin K-dependent protein family. Ucma seems to play crucial roles in processes related to mineralization, chondrogenic and osteogenic differentiation, and may be involved in skeletal homeostasis [3,4]. It is associated with the BMP-2-SMAD1/5/8 osteo/chondrogenic signalling pathway [1].
In Ucma-deficient mouse models, despite previous in vitro implications in calcification and ossification, normal skeletal and cartilage development was not affected [2]. However, VSMCs from Ucma/GRP-/-mice showed increased mineralisation and up-regulation of osteo/chondrogenic markers, along with decreased expression of the mineralisation inhibitor MGP, indicating that Ucma/GRP is an inhibitor of mineralisation. Ucma/GRP also inhibits the BMP-2-SMAD1/5/8 osteo/chondrogenic signalling pathway in VSMCs treated with elevated phosphate concentrations [1].
In conclusion, model-based research, especially using Ucma-deficient mouse models, has revealed that Ucma plays a significant role in regulating osteo/chondrogenic differentiation and phosphate-induced mineralisation of VSMCs. Although not necessary for normal skeletal development in mice, it may be involved in skeletal homeostasis and could be relevant in disease conditions related to vascular and soft-tissue calcification, such as in chronic kidney disease patients where vascular calcification is prevalent [1,2].
References:
1. Willems, Brecht A, Furmanik, Malgorzata, Caron, Marjolein M J, Reutelingsperger, Chris P M, Schurgers, Leon J. 2018. Ucma/GRP inhibits phosphate-induced vascular smooth muscle cell calcification via SMAD-dependent BMP signalling. In Scientific reports, 8, 4961. doi:10.1038/s41598-018-23353-y. https://pubmed.ncbi.nlm.nih.gov/29563538/
2. Eitzinger, Nicole, Surmann-Schmitt, Cordula, Bösl, Michael, von der Mark, Klaus, Stock, Michael. 2011. Ucma is not necessary for normal development of the mouse skeleton. In Bone, 50, 670-80. doi:10.1016/j.bone.2011.11.017. https://pubmed.ncbi.nlm.nih.gov/22155508/
3. Bordoloi, Jijnasa, Dihingia, Anjum, Kalita, Jatin, Manna, Prasenjit. 2018. Implication of a novel vitamin K dependent protein, GRP/Ucma in the pathophysiological conditions associated with vascular and soft tissue calcification, osteoarthritis, inflammation, and carcinoma. In International journal of biological macromolecules, 113, 309-316. doi:10.1016/j.ijbiomac.2018.02.150. https://pubmed.ncbi.nlm.nih.gov/29499263/
4. Surmann-Schmitt, Cordula, Dietz, Uwe, Kireva, Trayana, von der Mark, Klaus, Stock, Michael. 2007. Ucma, a novel secreted cartilage-specific protein with implications in osteogenesis. In The Journal of biological chemistry, 283, 7082-93. doi:. https://pubmed.ncbi.nlm.nih.gov/18156182/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen