C57BL/6NCya-Cdk12em1/Cya
Common Name:
Cdk12-KO
Product ID:
S-KO-12958
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cdk12-KO
Strain ID
KOCMP-69131-Cdk12-B6N-VA
Gene Name
Product ID
S-KO-12958
Gene Alias
1810022J16Rik; Crk7; Crkrs; D11Ertd752e; Pksc
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Cdk12em1/Cya mice (Catalog S-KO-12958) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000107538
NCBI RefSeq
NM_001109626
Target Region
Exon 4
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Cyclin-dependent kinase 12 (Cdk12) is a member of the CDK family of serine/threonine protein kinases, which associates with cyclin K to exert its functions [5]. It is crucial in regulating gene transcription, mRNA processing, and translation, and plays a significant role in diverse biological processes such as cell cycle progression, cell proliferation, and DNA damage response (DDR) [1,2,5].
In prostate cancer, biallelic loss of Cdk12 defines a metastatic castration-resistant prostate cancer (mCRPC) subtype. Cdk12 ablation in murine prostate epithelium can induce preneoplastic lesions with lymphocytic infiltration, and concurrent Cdk12/Trp53 ablation promotes proliferation of prostate-derived organoids. Also, Cdk12-mutant organoids and patient-derived xenografts are sensitive to inhibition or degradation of the paralog kinase, CDK13 [4]. In triple-negative breast cancer, inhibition or loss of Cdk12/CDK13 triggers intronic polyadenylation site cleavage that suppresses the expression of core DNA damage response proteins, leading to a "BRCAness" phenotype [3].
In conclusion, Cdk12 is essential for multiple biological functions including gene regulation and cell-cycle-related processes. The gene knockout (KO) and conditional knockout (CKO) mouse models have been instrumental in revealing its role in prostate and breast cancers, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Emadi, Fatemeh, Teo, Theodosia, Rahaman, Muhammed H, Wang, Shudong. 2020. CDK12: a potential therapeutic target in cancer. In Drug discovery today, 25, 2257-2267. doi:10.1016/j.drudis.2020.09.035. https://pubmed.ncbi.nlm.nih.gov/33038524/
2. Liang, Shujing, Hu, Lifang, Wu, Zixiang, Xu, Xia, Qian, Airong. 2020. CDK12: A Potent Target and Biomarker for Human Cancer Therapy. In Cells, 9, . doi:10.3390/cells9061483. https://pubmed.ncbi.nlm.nih.gov/32570740/
3. Quereda, Victor, Bayle, Simon, Vena, Francesca, Roush, William R, Duckett, Derek R. 2019. Therapeutic Targeting of CDK12/CDK13 in Triple-Negative Breast Cancer. In Cancer cell, 36, 545-558.e7. doi:10.1016/j.ccell.2019.09.004. https://pubmed.ncbi.nlm.nih.gov/31668947/
4. Tien, Jean Ching-Yi, Luo, Jie, Chang, Yu, Ding, Ke, Chinnaiyan, Arul M. 2024. CDK12 loss drives prostate cancer progression, transcription-replication conflicts, and synthetic lethality with paralog CDK13. In Cell reports. Medicine, 5, 101758. doi:10.1016/j.xcrm.2024.101758. https://pubmed.ncbi.nlm.nih.gov/39368479/
5. Tang, Ruijun, Liu, Jing, Li, Shuyao, Yuan, Kai, Shao, Hao. 2022. A patent and literature review of CDK12 inhibitors. In Expert opinion on therapeutic patents, 32, 1055-1065. doi:10.1080/13543776.2022.2126765. https://pubmed.ncbi.nlm.nih.gov/36120913/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen