Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Polr2dem1/Cya
Common Name:
Polr2d-KO
Product ID:
S-KO-13003
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Polr2d-KO
Strain ID
KOCMP-69241-Polr2d-B6J-VA
Gene Name
Polr2d
Product ID
S-KO-13003
Gene Alias
2310002G05Rik; 2610028L19Rik; HSRBP4; RBP4
Background
C57BL/6JCya
NCBI ID
69241
Modification
Conventional knockout
Chromosome
18
Phenotype
MGI:1916491
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Polr2dem1/Cya mice (Catalog S-KO-13003) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025106
NCBI RefSeq
NM_027002
Target Region
Exon 1~4
Size of Effective Region
~7.0 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Polr2d, also known as RPB4, encodes a subunit of DNA-directed RNA polymerase II (pol II). The pol II enzyme, composed of ten core and two dissociable subunits, is crucial for transcribing DNA into RNA. The dissociable subcomplex of pol II, where Polr2d is a part, is a heterodimer with Rpb7/Polr2g. Polr2d is involved in pol II-mediated RNA synthesis and various mRNA regulations like pre-mRNA splicing, nuclear export of mRNAs, and mRNA decay [1].

In zebrafish, polr2d-deficient mutants exhibited progressive delay of somitogenesis starting at 11 h post-fertilization, increased cell death at 15 hpf, hypoplasia such as small eye and cardiac edema by 48 hpf, and premature death by 60 hpf. RT-qPCR showed diminished expression of housekeeping and zygotic genes in these mutants, indicating that Polr2d is indispensable for vertebrate development [1]. In mouse oocyte maturation, knockdown of Mat2b led to aberrant expression of Polr2d along with other maternal transcripts, and Mat2b was shown to play an important role in mouse oocyte maturation through the MAPK signaling pathway [2]. In pan-cancer analysis, POLR2D was identified as a commonly overexpressed and prognostic gene, and its knockdown decreased cell proliferation, suggesting a role in cancer-related cell growth [3]. In colon adenocarcinoma, POLR2D was part of a 16-metabolic-gene model that could predict patient survival prognosis [4].

In summary, Polr2d is essential for vertebrate development, playing key roles in processes like somitogenesis and maintaining proper gene expression. In addition, it is involved in mouse oocyte maturation through related signaling pathways. Its abnormal expression is associated with cancer cell growth and can be used as a biomarker for predicting colon adenocarcinoma prognosis. The study of Polr2d knockout models in zebrafish and other findings related to its expression changes in mouse oocytes and cancer cells have deepened our understanding of its functions in development and disease.

References:

1. Maeta, Masanari, Kataoka, Miku, Nishiya, Yusuke, Kashima, Makoto, Hirata, Hiromi. 2020. RNA polymerase II subunit D is essential for zebrafish development. In Scientific reports, 10, 13213. doi:10.1038/s41598-020-70110-1. https://pubmed.ncbi.nlm.nih.gov/32764610/

2. An, Quanli, Sun, Hongzheng, Zhang, Jun, Zhang, Yong, Su, Jianmin. 2020. Methionine Adenosyltransferase 2β Participates in Mouse Oocyte Maturation by Regulating the MAPK Pathway. In Reproductive sciences (Thousand Oaks, Calif.), 27, 163-171. doi:10.1007/s43032-019-00015-6. https://pubmed.ncbi.nlm.nih.gov/32046373/

3. Bhandari, Nikita, Acharya, Disha, Chatterjee, Annesha, Malakar, Pushkar, Shukla, Sudhanshu K. 2023. Pan-cancer integrated bioinformatic analysis of RNA polymerase subunits reveal RNA Pol I member CD3EAP regulates cell growth by modulating autophagy. In Cell cycle (Georgetown, Tex.), 22, 1986-2002. doi:10.1080/15384101.2023.2265676. https://pubmed.ncbi.nlm.nih.gov/37795959/

4. Zhao, Fuqiang, Liu, Yanlong, Gu, Xinyue, Song, Chengxin, Cui, Binbin. . Identification of sixteen metabolic genes as potential biomarkers for colon adenocarcinoma. In Journal of B.U.ON. : official journal of the Balkan Union of Oncology, 26, 1252-1259. doi:. https://pubmed.ncbi.nlm.nih.gov/34564978/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest