C57BL/6JCya-Tmem127em1/Cya
Common Name
Tmem127-KO
Product ID
S-KO-13073
Backgroud
C57BL/6JCya
Strain ID
KOCMP-69470-Tmem127-B6J-VA
When using this mouse strain in a publication, please cite “Tmem127-KO Mouse (Catalog S-KO-13073) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tmem127-KO
Strain ID
KOCMP-69470-Tmem127-B6J-VA
Gene Name
Product ID
S-KO-13073
Gene Alias
2310003P10Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000035871
NCBI RefSeq
NM_175145
Target Region
Exon 3~4
Size of Effective Region
~5.1 kb
Overview of Gene Research
TMEM127, a transmembrane protein-encoding gene, is a tumor suppressor. It is involved in multiple biological processes. TMEM127 is part of the mTORC1 lysosomal nutrient-sensing complex, regulating mTORC1 signaling in response to amino acids [3]. It also contributes to the ubiquitin system, influencing the cellular positioning, trafficking, and lysosome-mediated degradation of the tyrosine kinase receptor RET [1].
In pheochromocytoma, TMEM127-mutant tumors share precursor cells and transcription regulatory elements with RET-mutated pheochromocytomas [1]. Loss of TMEM127 in human cells and mouse knockout models leads to RET accumulation and increased signaling, promoting cell proliferation and tumor burden [1]. Whole-body Tmem127 knockout mice have decreased adiposity, maintain insulin sensitivity, and show low hepatic fat deposition and peripheral glucose clearance after a high-fat diet. Liver-specific and adipose-specific Tmem127 deletion also affects glucose and lipid homeostasis in a tissue-specific manner [2].
In conclusion, TMEM127 functions as a tumor suppressor and is involved in nutrient-sensing related to glucose/lipid homeostasis. Mouse knockout models have revealed its role in pheochromocytoma development and metabolic regulation, providing insights into disease mechanisms and potential therapeutic targets for these conditions.
References:
1. Guo, Qianjin, Cheng, Zi-Ming, Gonzalez-Cantú, Hector, Aguiar, Ricardo C T, Dahia, Patricia L M. 2023. TMEM127 suppresses tumor development by promoting RET ubiquitination, positioning, and degradation. In Cell reports, 42, 113070. doi:10.1016/j.celrep.2023.113070. https://pubmed.ncbi.nlm.nih.gov/37659079/
2. Srikantan, Subramanya, Deng, Yilun, Cheng, Zi-Ming, Aguiar, Ricardo C T, Dahia, Patricia L M. 2019. The tumor suppressor TMEM127 regulates insulin sensitivity in a tissue-specific manner. In Nature communications, 10, 4720. doi:10.1038/s41467-019-12661-0. https://pubmed.ncbi.nlm.nih.gov/31624249/
3. Deng, Yilun, Qin, Yuejuan, Srikantan, Subramanya, Wang, Exing, Dahia, Patricia L M. . The TMEM127 human tumor suppressor is a component of the mTORC1 lysosomal nutrient-sensing complex. In Human molecular genetics, 27, 1794-1808. doi:10.1093/hmg/ddy095. https://pubmed.ncbi.nlm.nih.gov/29547888/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.