C57BL/6NCya-Dnah17em1/Cya
Common Name:
Dnah17-KO
Product ID:
S-KO-13218
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dnah17-KO
Strain ID
KOCMP-69926-Dnah17-B6N-VA
Gene Name
Product ID
S-KO-13218
Gene Alias
2810003K23Rik; Dnahc17; Dnahcl1; Gm1178; mKIAA3028
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Dnah17em1/Cya mice (Catalog S-KO-13218) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000084803
NCBI RefSeq
NM_001167746
Target Region
Exon 2~11
Size of Effective Region
~7.8 kb
Detailed Document
Overview of Gene Research
DNAH17, dynein axonemal heavy chain 17, is crucial for spermatogenesis and male fertility [2,3,4]. It is specifically expressed in testes and localized to sperm flagella [4]. Dysfunction in DNAH17 can lead to sperm flagellum defects, which are associated with asthenozoospermia and male infertility [1,3,4].
Knockout Dnah17 rats generated by Nuclease technology gene editing showed that homozygous male rats were infertile, with significantly decreased sperm count and increased apoptosis in testes, suggesting that DNAH17 is essential for spermatogenesis in male rats [2]. In humans, various DNAH17 mutations have been identified in patients with multiple morphological abnormalities of the sperm flagella (MMAF) phenotype, which is a severe form of asthenozoospermia. These patients generally have worse pregnancy outcomes following intracytoplasmic sperm injection (ICSI), but ICSI combined with assisted oocyte activation (AOA) may improve the outcome of assisted reproductive techniques for men with DNAH17 variants [1].
In conclusion, DNAH17 is essential for spermatogenesis and male fertility. Studies on knockout rat models and human patients with DNAH17 mutations have revealed its crucial role in sperm development and the implications for male infertility. These findings provide important insights for understanding the genetic basis of male infertility and for improving assisted reproductive techniques [1,2].
References:
1. Song, Bing, Yang, Tianjin, Shen, Qunshan, Cao, Yunxia, He, Xiaojin. 2023. Novel mutations in DNAH17 cause sperm flagellum defects and their influence on ICSI outcome. In Journal of assisted reproduction and genetics, 40, 2485-2492. doi:10.1007/s10815-023-02897-7. https://pubmed.ncbi.nlm.nih.gov/37574497/
2. Chen, Liling, Ouyang, Jiamin, Li, Xueqing, Liao, Yuandongs, Zhang, Qingjiong. . DNAH17 is essential for rat spermatogenesis and fertility. In Journal of genetics, 100, . doi:. https://pubmed.ncbi.nlm.nih.gov/33764336/
3. Sha, Yanwei, Wei, Xiaoli, Ding, Lu, Zhang, Yi, Ji, Zhiyong. 2019. DNAH17 is associated with asthenozoospermia and multiple morphological abnormalities of sperm flagella. In Annals of human genetics, 84, 271-279. doi:10.1111/ahg.12369. https://pubmed.ncbi.nlm.nih.gov/31841227/
4. Zhang, Beibei, Ma, Hui, Khan, Teka, Zhang, Yuanwei, Shi, Qinghua. . A DNAH17 missense variant causes flagella destabilization and asthenozoospermia. In The Journal of experimental medicine, 217, . doi:10.1084/jem.20182365. https://pubmed.ncbi.nlm.nih.gov/31658987/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen