C57BL/6JCya-Bbxem1/Cya
Common Name:
Bbx-KO
Product ID:
S-KO-13361
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Bbx-KO
Strain ID
KOCMP-70508-Bbx-B6J-VA
Gene Name
Product ID
S-KO-13361
Gene Alias
5530401J07Rik; 5730403O13Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bbxem1/Cya mice (Catalog S-KO-13361) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000138166
NCBI RefSeq
NM_027444
Target Region
Exon 5~6
Size of Effective Region
~8.6 kb
Detailed Document
Overview of Gene Research
Bbx, short for bobby sox homolog, is a transcription factor encoding zinc finger protein. Bbx proteins are key factors in regulatory networks controlling various plant growth and developmental processes, including seedling photomorphogenesis, photoperiodic regulation of flowering, shade avoidance, and responses to biotic and abiotic stresses [1,2,3,4,5,7,8,9]. They are involved in light and hormonal regulation pathways in plants [3,4,5]. In animals, Bbx is related to phosphate homeostasis [6].
In mice, Bbx knockout (Bbx -/-) led to increased FGF23 expression, low phosphate levels in the serum, and skeletal abnormalities like low bone mineral density, reduced bone volume, and short and weak bones associated with low bone formation. Osteocyte-specific deletion of Bbx also resulted in similar phenotypes [6].
In conclusion, Bbx plays crucial roles in plants in growth, development, and stress responses, and in mice, it is essential for regulating phosphate homeostasis and bone development. The Bbx -/- mouse model has provided valuable insights into the role of Bbx in bone-related conditions.
References:
1. Talar, Urszula, Kiełbowicz-Matuk, Agnieszka. 2021. Beyond Arabidopsis: BBX Regulators in Crop Plants. In International journal of molecular sciences, 22, . doi:10.3390/ijms22062906. https://pubmed.ncbi.nlm.nih.gov/33809370/
2. Yang, Ning, Cong, Qing, Cheng, Longjun. . [BBX transcriptional factors family in plants-a review]. In Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 36, 666-677. doi:10.13345/j.cjb.190302. https://pubmed.ncbi.nlm.nih.gov/32347061/
3. Bandara, Wathsala W, Wijesundera, W S S, Hettiarachchi, Chamari. 2022. Rice and Arabidopsis BBX proteins: toward genetic engineering of abiotic stress resistant crops. In 3 Biotech, 12, 164. doi:10.1007/s13205-022-03228-w. https://pubmed.ncbi.nlm.nih.gov/36092969/
4. Cao, Jing, Yuan, Jiale, Zhang, Yingli, Niu, Rui, Lin, Fang. 2023. Multi-layered roles of BBX proteins in plant growth and development. In Stress biology, 3, 1. doi:10.1007/s44154-022-00080-z. https://pubmed.ncbi.nlm.nih.gov/37676379/
5. Gangappa, Sreeramaiah N, Botto, Javier F. 2014. The BBX family of plant transcription factors. In Trends in plant science, 19, 460-70. doi:10.1016/j.tplants.2014.01.010. https://pubmed.ncbi.nlm.nih.gov/24582145/
6. Lee, Su Jeong, Kim, Ju Ang, Ihn, Hye Jung, Kim, Jung-Eun, Park, Eui Kyun. 2024. The transcription factor BBX regulates phosphate homeostasis through the modulation of FGF23. In Experimental & molecular medicine, 56, 2436-2448. doi:10.1038/s12276-024-01341-9. https://pubmed.ncbi.nlm.nih.gov/39482539/
7. Xuefen, Du, Wei, Xiaohong, Wang, Baoqiang, Xian, Wang, Jincheng, Luo. 2022. Genome-wide identification and expression pattern analysis of quinoa BBX family. In PeerJ, 10, e14463. doi:10.7717/peerj.14463. https://pubmed.ncbi.nlm.nih.gov/36523472/
8. Sarmiento, Felipe. 2013. The BBX subfamily IV: additional cogs and sprockets to fine-tune light-dependent development. In Plant signaling & behavior, 8, e23831. doi:10.4161/psb.23831. https://pubmed.ncbi.nlm.nih.gov/23425851/
9. Chang, Yingying, Sun, Haoyuan, Liu, Shiyu, Li, Mingjun, Zhao, Xiting. 2023. Identification of BBX gene family and its function in the regulation of microtuber formation in yam. In BMC genomics, 24, 354. doi:10.1186/s12864-023-09406-1. https://pubmed.ncbi.nlm.nih.gov/37365511/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen