C57BL/6JCya-Rbpms2em1/Cya
Common Name:
Rbpms2-KO
Product ID:
S-KO-13774
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Rbpms2-KO
Strain ID
KOCMP-71973-Rbpms2-B6J-VA
Gene Name
Product ID
S-KO-13774
Gene Alias
2400008B06Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rbpms2em1/Cya mice (Catalog S-KO-13774) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000055844
NCBI RefSeq
NM_028030
Target Region
Exon 2~5
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Rbpms2, an RNA-binding protein, is a member of the RNA-recognition-motif-containing protein family. It plays crucial roles in multiple biological processes. In the heart, it is a myocardial-enriched splicing regulator essential for cardiac function, involving in myofibril organization and calcium handling [1]. In zebrafish, it acts as a binary fate-switch in early gonocytes, promoting female fate by repressing testis factors and promoting oocyte factors through the mTorc1 signaling pathway [2,4].
Gene knockout studies have been instrumental in revealing its functions. Zebrafish rbpms2 knockout embryos display early cardiac dysfunction with reduced ejection fraction, myofibril disarray, and calcium handling abnormalities, similar phenotypes are also seen in RBPMS2-deficient human cardiomyocytes [1]. In zebrafish, rbpms2 mutants initially have early oocytes, but definitive oogenesis fails during sexual differentiation, and they develop as fertile males [3]. In mouse, maternal RBPMS2 knockdown inhibits embryo development from the morula to the blastocyst stage by impairing the BMP pathway and E-cadherin localization [5]. In Japanese flounder, siRNA interference and overexpression of Rbpms2 in gonadal primary cells showed its role in repressing male-related genes and promoting female-related genes [6].
In summary, Rbpms2 is essential for cardiac function, sexual differentiation in zebrafish and Japanese flounder, and early mouse embryo development. The gene knockout models in different species have significantly contributed to understanding its functions in these biological processes, providing insights into potential mechanisms underlying heart diseases and reproductive disorders.
References:
1. Akerberg, Alexander A, Trembley, Michael, Butty, Vincent, Burns, Caroline E, Burns, C Geoffrey. 2022. RBPMS2 Is a Myocardial-Enriched Splicing Regulator Required for Cardiac Function. In Circulation research, 131, 980-1000. doi:10.1161/CIRCRESAHA.122.321728. https://pubmed.ncbi.nlm.nih.gov/36367103/
2. Wilson, Miranda L, Romano, Shannon N, Khatri, Nitya, Draper, Bruce W, Marlow, Florence L. 2024. Rbpms2 promotes female fate upstream of the nutrient sensing Gator2 complex component Mios. In Nature communications, 15, 5248. doi:10.1038/s41467-024-49613-2. https://pubmed.ncbi.nlm.nih.gov/38898112/
3. Kaufman, Odelya H, Lee, KathyAnn, Martin, Manon, Rothhämel, Sophie, Marlow, Florence L. 2018. rbpms2 functions in Balbiani body architecture and ovary fate. In PLoS genetics, 14, e1007489. doi:10.1371/journal.pgen.1007489. https://pubmed.ncbi.nlm.nih.gov/29975683/
4. Wilson, Miranda L, Romano, Shannon N, Khatri, Nitya, Draper, Bruce W, Marlow, Florence L. 2024. Rbpms2 promotes female fate upstream of the nutrient sensing Gator2 complex component, Mios. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.01.25.577235. https://pubmed.ncbi.nlm.nih.gov/38328218/
5. Zhou, Cheng, Zheng, Lu, Teng, Hui, Chen, Li, Yao, Bing. 2023. Maternal RNA binding protein with multiple splicing 2 (RBPMS2) is involved in mouse blastocyst formation through the bone morphogenetic protein pathway. In Reproductive biomedicine online, 47, 103238. doi:10.1016/j.rbmo.2023.05.010. https://pubmed.ncbi.nlm.nih.gov/37573751/
6. Liu, Cui, Zhang, Longsheng, Xia, You, Wu, Jikui, Zhang, Junling. 2024. Identification, expression, and function analysis of Rbpms2 splicing variants in Japanese flounder gonad. In General and comparative endocrinology, 359, 114628. doi:10.1016/j.ygcen.2024.114628. https://pubmed.ncbi.nlm.nih.gov/39414089/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen