C57BL/6NCya-Osgepl1em1/Cya
Common Name:
Osgepl1-KO
Product ID:
S-KO-13824
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Osgepl1-KO
Strain ID
KOCMP-72085-Osgepl1-B6N-VA
Gene Name
Product ID
S-KO-13824
Gene Alias
2610001M19Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Osgepl1em1/Cya mice (Catalog S-KO-13824) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027265
NCBI RefSeq
NM_001285839
Target Region
Exon 3~4
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Osgepl1, an ortholog of OSGEP in mitochondria, is a crucial enzyme for the formation of N6-threonylcarbamoyladenosine at A37 (t6A37) in mitochondrial tRNAs [1,2,3,4]. t6A37 modification is essential for translational accuracy and fidelity, and Osgepl1 is involved in the mitochondrial translation pathway [1,2,3,4]. Its proper function is of great significance for mitochondrial gene expression and overall cell function [1,4]. Genetic models, such as gene knockout in cells and mice, are valuable for studying Osgepl1.
In HEK293T cells, deletion of Osgepl1 led to t6A37 hypomodification, which selectively stimulated other tRNA modifications like N1-methyladenosine at A9 (m1A9) and N2-methylguanosine at G10 (m2G10), reduced the aminoacylation of mitochondrial tRNAThr and tRNALys, impaired translation efficiency, and triggered translational infidelity. This also caused alterations in mitochondrial structure, function, and activated the mitochondrial unfolded protein response [1]. In Osgepl1 deletion mice, disruption to mitochondrial translation was evident, but no observable deficiency was seen under physiological conditions in the heart, despite the high Osgepl1 expression in the heart [1]. In addition, in vitro experiments showed that OSGEPL1 is a monomer and its activity is affected by acetylation, and specific tRNA sequences are fine-tuned for different modification levels [2].
In summary, Osgepl1 is vital for maintaining mitochondrial translation efficiency and quality control through t6A37 modification [1]. The gene knockout studies in cells and mice have revealed its role in mitochondrial-related biological processes, though the lack of physiological deficiency in the heart of knockout mice indicates potential compensatory mechanisms. Understanding Osgepl1 may provide insights into mitochondrial-related diseases and translational regulation [1].
References:
1. Zhang, Yong, Zhou, Jing-Bo, Yin, Yue, Wang, En-Duo, Zhou, Xiao-Long. . Multifaceted roles of t6A biogenesis in efficiency and fidelity of mitochondrial gene expression. In Nucleic acids research, 52, 3213-3233. doi:10.1093/nar/gkae013. https://pubmed.ncbi.nlm.nih.gov/38227555/
2. Zhou, Jing-Bo, Wang, Yong, Zeng, Qi-Yu, Wang, En-Duo, Zhou, Xiao-Long. . Molecular basis for t6A modification in human mitochondria. In Nucleic acids research, 48, 3181-3194. doi:10.1093/nar/gkaa093. https://pubmed.ncbi.nlm.nih.gov/32047918/
3. Su, Chenchen, Jin, Mengqi, Zhang, Wenhua. 2022. Conservation and Diversification of tRNA t6A-Modifying Enzymes across the Three Domains of Life. In International journal of molecular sciences, 23, . doi:10.3390/ijms232113600. https://pubmed.ncbi.nlm.nih.gov/36362385/
4. Lin, Huan, Miyauchi, Kenjyo, Harada, Tai, Sakaguchi, Yuriko, Suzuki, Tsutomu. 2018. CO2-sensitive tRNA modification associated with human mitochondrial disease. In Nature communications, 9, 1875. doi:10.1038/s41467-018-04250-4. https://pubmed.ncbi.nlm.nih.gov/29760464/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen