C57BL/6JCya-Mir335em1/Cya
Common Name:
Mir335-KO
Product ID:
S-KO-13932
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Mir335-KO
Strain ID
KOCMP-723930-Mir335-B6J-VA
Gene Name
Product ID
S-KO-13932
Gene Alias
Mirn335; mir-335; mmu-mir-335
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mir335em1/Cya mice (Catalog S-KO-13932) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000083567
NCBI RefSeq
NR_029900
Target Region
Exon 1
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Mir335, a microRNA, is involved in multiple biological processes. It plays a role in maintaining the balance of insulin exocytosis in pancreatic β -cells, and is associated with metabolic homeostasis pathways [3]. In addition, it may function as an oncogene or tumor-suppressor gene in cancers, influencing tumor initiation, development, and metastasis [5]. Genetic models, such as gene knockout mouse models, can be valuable for further exploring its functions.
In breast cancer, reduced Mir335 expression is associated with poor prognosis, indicating its potential as a biomarker [1]. In steroid-associated osteonecrosis, tetrahedral DNA nanostructures delivering MiR335-5p can repair bone defects by targeting DKK1 and upregulating the Wnt signaling pathway [2]. In paediatric acute lymphoblastic leukaemia, exogenous expression of MIR335 in ALL cells increases sensitization to prednisolone-mediated apoptosis, and MAPK1 is a novel target of MIR335 [4].
In conclusion, Mir335 is crucial in metabolism-related biological functions and has implications in various disease areas. Its study using gene knockout models has revealed its significance in breast cancer prognosis, bone defect repair in steroid-associated osteonecrosis, and the treatment of paediatric acute lymphoblastic leukaemia. These findings provide insights into potential therapeutic strategies for these diseases.
References:
1. Bertoli, Gloria, Cava, Claudia, Castiglioni, Isabella. 2015. MicroRNAs: New Biomarkers for Diagnosis, Prognosis, Therapy Prediction and Therapeutic Tools for Breast Cancer. In Theranostics, 5, 1122-43. doi:10.7150/thno.11543. https://pubmed.ncbi.nlm.nih.gov/26199650/
2. Li, Donghai, Yang, Zhouyuan, Luo, Yue, Tian, Meng, Kang, Pengde. 2021. Delivery of MiR335-5p-Pendant Tetrahedron DNA Nanostructures Using an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis. In Advanced healthcare materials, 11, e2101412. doi:10.1002/adhm.202101412. https://pubmed.ncbi.nlm.nih.gov/34694067/
3. Vienberg, S, Geiger, J, Madsen, S, Dalgaard, L T. 2016. MicroRNAs in metabolism. In Acta physiologica (Oxford, England), 219, 346-361. doi:10.1111/apha.12681. https://pubmed.ncbi.nlm.nih.gov/27009502/
4. Yan, Junli, Jiang, Nan, Huang, Gaofeng, Yeoh, Allen E J, Chng, Wee-Joo. 2013. Deregulated MIR335 that targets MAPK1 is implicated in poor outcome of paediatric acute lymphoblastic leukaemia. In British journal of haematology, 163, 93-103. doi:10.1111/bjh.12489. https://pubmed.ncbi.nlm.nih.gov/23888996/
5. Ye, Lingling, Wang, Fen, Wu, Hao, Wang, Jinyan, Zhang, Quan An. 2021. Functions and Targets of miR-335 in Cancer. In OncoTargets and therapy, 14, 3335-3349. doi:10.2147/OTT.S305098. https://pubmed.ncbi.nlm.nih.gov/34045870/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen