C57BL/6JCya-Mir200cem1/Cya
Common Name
Mir200c-KO
Product ID
S-KO-13934
Backgroud
C57BL/6JCya
Strain ID
KOCMP-723944-Mir200c-B6J-VA
When using this mouse strain in a publication, please cite “Mir200c-KO Mouse (Catalog S-KO-13934) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Mir200c-KO
Strain ID
KOCMP-723944-Mir200c-B6J-VA
Gene Name
Product ID
S-KO-13934
Gene Alias
Mirn200c, mir-200c
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000083528
NCBI RefSeq
NR_029792
Target Region
Exon 1
Size of Effective Region
~0.3 kb
Overview of Gene Research
Mir200c is a microRNA that has been implicated in various biological functions and disease-related processes. It plays roles in post-transcriptional regulation, affecting the expression of target genes involved in multiple pathways, which are crucial for maintaining normal cellular functions and are associated with diseases such as cancer and intestinal inflammation [1-10].
In intestinal inflammation, IL1B up-regulates Mir200C-3p, which degrades occludin mRNA, increasing intestinal tight junction permeability. In mouse models, antagomiR-200C (an antagonist to MIR200C-3p) prevents the decrease in occludin in enterocytes and maintains the tight junction barrier [1]. In prostate cancer, over-expression of miR200c suppresses cell growth by targeting insulin receptor substrate 1 (IRS1) [2]. In colorectal cancer, miR200c can attenuate P-gp-mediated multidrug resistance (MDR) and metastasis by targeting the JNK2/c-Jun signaling pathway. In an orthotopic MDR colorectal cancer mouse model, over-expression of miR200c effectively inhibits tumor growth and metastasis [3].
In summary, Mir200c is involved in post-transcriptional regulation of multiple genes, affecting biological processes relevant to disease development. Studies using mouse models, such as those with modulation of Mir200c expression, have revealed its roles in intestinal inflammation, prostate cancer, and colorectal cancer, providing insights into potential therapeutic strategies for these diseases [1,2,3].
References:
1. Rawat, Manmeet, Nighot, Meghali, Al-Sadi, Rana, Koltun, Walter, Ma, Thomas Y. 2020. IL1B Increases Intestinal Tight Junction Permeability by Up-regulation of MIR200C-3p, Which Degrades Occludin mRNA. In Gastroenterology, 159, 1375-1389. doi:10.1053/j.gastro.2020.06.038. https://pubmed.ncbi.nlm.nih.gov/32569770/
2. Su, Wenjing, Xu, Miao, Chen, Xueqin, Huang, Lei, Zhou, Qiao. 2015. MiR200c targets IRS1 and suppresses prostate cancer cell growth. In The Prostate, 75, 855-62. doi:10.1002/pros.22968. https://pubmed.ncbi.nlm.nih.gov/25683382/
3. Sui, Hua, Cai, Guo-Xiang, Pan, Shu-Fang, Zhu, Hui-Rong, Li, Qi. 2014. miR200c attenuates P-gp-mediated MDR and metastasis by targeting JNK2/c-Jun signaling pathway in colorectal cancer. In Molecular cancer therapeutics, 13, 3137-51. doi:10.1158/1535-7163.MCT-14-0167. https://pubmed.ncbi.nlm.nih.gov/25205654/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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