C57BL/6JCya-Ift70bem1/Cya
Common Name
Ift70b-KO
Product ID
S-KO-13939
Backgroud
C57BL/6JCya
Strain ID
KOCMP-72421-Ift70b-B6J-VA
Status
When using this mouse strain in a publication, please cite “Ift70b-KO Mouse (Catalog S-KO-13939) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Ift70b-KO
Strain ID
KOCMP-72421-Ift70b-B6J-VA
Gene Name
Product ID
S-KO-13939
Gene Alias
Tcc30b, Ttc30b, 2510042P03Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000099996
NCBI RefSeq
NM_028235
Target Region
Exon 1
Size of Effective Region
~2.7 kb
Overview of Gene Research
Ift70b is one of the genes related to the IFT-B complex in the intraflagellar transport (IFT) machinery. The IFT-B complex is crucial for anterograde ciliary protein trafficking, and ciliogenesis is an essential biological process it is associated with [1].
In Ift70-knockout (KO) studies, Ift70A and Ift70B double-KO cells did not form cilia, despite Ift70 being peripherally associated with the IFT-B complex via the IFT52-IFT88 dimer. Other IFT-B subunits assembled at the ciliary base in the absence of Ift70. Exogenous expression of either Ift70A or Ift70B could restore the ciliogenesis defect in Ift70-KO cells, indicating redundant roles. Deletion of certain structural elements of Ift70A, like the first tetratricopeptide repeat (TPR) or the short helix (α36), reduced its interaction with the IFT52-IFT88 dimer and could not restore ciliogenesis in KO cells [1].
In conclusion, Ift70b, along with Ift70A, plays an essential role in ciliogenesis. The Ift70-KO models have revealed that its interaction with the IFT52-IFT88 dimer is crucial for this function. Understanding Ift70b's role in ciliogenesis may contribute to insights in ciliopathy-related disease areas [1].
References:
1. Takei, Ryota, Katoh, Yohei, Nakayama, Kazuhisa. 2018. Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis. In Biology open, 7, . doi:10.1242/bio.033241. https://pubmed.ncbi.nlm.nih.gov/29654116/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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