C57BL/6NCya-Pard3bem1/Cya
Common Name:
Pard3b-KO
Product ID:
S-KO-14037
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pard3b-KO
Strain ID
KOCMP-72823-Pard3b-B6N-VA
Gene Name
Product ID
S-KO-14037
Gene Alias
1810008K04Rik; 2010002N16Rik; 2810455B10Rik; Als2cr19; PAR3B; PAR3L; PAR3beta
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Pard3bem1/Cya mice (Catalog S-KO-14037) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000075374
NCBI RefSeq
NM_001081050
Target Region
Exon 3
Size of Effective Region
~0.2 kb
Detailed Document
Overview of Gene Research
PARD3B, a member of the Par-3/Baz family of polarity determinants, is highly conserved across metazoans. In vertebrates, it is specifically expressed in basal layer stem cells of stratified squamous epithelia where it localizes strictly at adherens junctions in many epithelia. It may contribute to clustering of E-cadherin, signalling from adherens junctions via Src family kinases, or mitotic spindle orientation by adherens junctions in response to mechanical forces [3].
In glioblastoma multiforme (GBM), expressions of AR and PARD3B mRNA and proteins in human GBM tissues are upregulated compared to normal human brain tissues, and there is a highly positive correlation between them. The testosterone AR-PARD3B signaling axis contributes to GBM tumorigenesis and malignance through stimulating cell proliferation and colony formation, as suppressing AR activity attenuates testosterone-induced PARD3B gene expression, cell proliferation, and colony formation in human glioblastoma cells [1]. In intrahepatic cholangiocarcinoma (ICC), VIRMA facilitates ICC progression by epigenetically augmenting TMED2 and PARD3B mRNA stabilization. VIRMA-induced expression of PARD3B activates the Akt/GSK/β-catenin and MEK/ERK/Slug signaling pathways, promoting ICC proliferation and metastasis [2].
In conclusion, PARD3B is involved in cell polarity and adhesion, and plays significant roles in diseases such as GBM and ICC. Studies related to PARD3B, including those potentially using gene knockout or other loss-of-function models, help reveal its functions in specific disease-related biological processes, providing potential therapeutic targets for these diseases.
References:
1. Yang, Jr-Di, Chen, Jui-Tai, Liu, Shing-Hwa, Chen, Ruei-Ming. 2022. Contribution of the Testosterone Androgen Receptor-PARD3B Signaling Axis to Tumorigenesis and Malignance of Glioblastoma Multiforme through Stimulating Cell Proliferation and Colony Formation. In Journal of clinical medicine, 11, . doi:10.3390/jcm11164818. https://pubmed.ncbi.nlm.nih.gov/36013056/
2. Xu, Hongfa, Lin, Xiaowen, Li, Zhongliang, Zhan, Meixiao, He, Ke. 2023. VIRMA facilitates intrahepatic cholangiocarcinoma progression through epigenetic augmentation of TMED2 and PARD3B mRNA stabilization. In Journal of gastroenterology, 58, 925-944. doi:10.1007/s00535-023-02015-5. https://pubmed.ncbi.nlm.nih.gov/37391589/
3. Thompson, Barry J. 2021. Par-3 family proteins in cell polarity & adhesion. In The FEBS journal, 289, 596-613. doi:10.1111/febs.15754. https://pubmed.ncbi.nlm.nih.gov/33565714/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen