C57BL/6JCya-Fbxl22em1/Cya
Common Name
Fbxl22-KO
Product ID
S-KO-14319
Backgroud
C57BL/6JCya
Strain ID
KOCMP-74165-Fbxl22-B6J-VA
When using this mouse strain in a publication, please cite “Fbxl22-KO Mouse (Catalog S-KO-14319) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Fbxl22-KO
Strain ID
KOCMP-74165-Fbxl22-B6J-VA
Gene Name
Product ID
S-KO-14319
Gene Alias
1110004B15Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 9
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000056890
NCBI RefSeq
NM_175206
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Overview of Gene Research
Fbxl22, or F-box and leucine-rich protein 22, is an E3 ubiquitin ligase. It is involved in the ubiquitin-proteasome system, regulating protein degradation. In muscle, it has a role in muscle homeostasis and atrophy processes, and in the heart, it is crucial for maintaining contractile function by regulating sarcomeric protein turnover [2,3].
In skeletal muscle, overexpression of Fbxl22 isoforms in mice leads to myopathy/atrophy, suggesting its role in neurogenic muscle atrophy. Knockdown of Fbxl22 in MuRF1 KO mice muscles results in significant additive muscle sparing after denervation, indicating its importance in muscle mass regulation [2]. In cardiomyocytes, targeted knockdown of Fbxl22 in rats and zebrafish embryos causes the accumulation of α-actinin, severely impaired contractile function, and cardiomyopathy [3]. Also, in zebrafish heart regeneration, blocking AP-1 function leads to decreased chromatin accessibility at the fbxl22 locus, affecting sarcomere disassembly [1].
In conclusion, Fbxl22 is essential for muscle and cardiac function. Studies using gene-knockdown and over-expression models in mice and zebrafish have revealed its role in muscle atrophy and cardiac contractility. These findings have implications for understanding muscle-related and cardiac diseases, and potentially developing therapeutic strategies for these conditions.
References:
1. Beisaw, Arica, Kuenne, Carsten, Guenther, Stefan, Looso, Mario, Stainier, Didier Y R. 2020. AP-1 Contributes to Chromatin Accessibility to Promote Sarcomere Disassembly and Cardiomyocyte Protrusion During Zebrafish Heart Regeneration. In Circulation research, 126, 1760-1778. doi:10.1161/CIRCRESAHA.119.316167. https://pubmed.ncbi.nlm.nih.gov/32312172/
2. Hughes, David C, Baehr, Leslie M, Driscoll, Julia R, Waddell, David S, Bodine, Sue C. 2020. Identification and characterization of Fbxl22, a novel skeletal muscle atrophy-promoting E3 ubiquitin ligase. In American journal of physiology. Cell physiology, 319, C700-C719. doi:10.1152/ajpcell.00253.2020. https://pubmed.ncbi.nlm.nih.gov/32783651/
3. Spaich, Sebastian, Will, Rainer D, Just, Steffen, Rottbauer, Wolfgang, Frey, Norbert. 2012. F-box and leucine-rich repeat protein 22 is a cardiac-enriched F-box protein that regulates sarcomeric protein turnover and is essential for maintenance of contractile function in vivo. In Circulation research, 111, 1504-16. doi:10.1161/CIRCRESAHA.112.271007. https://pubmed.ncbi.nlm.nih.gov/22972877/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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