C57BL/6JCya-Ttc27em1/Cya
Common Name:
Ttc27-KO
Product ID:
S-KO-14333
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ttc27-KO
Strain ID
KOCMP-74196-Ttc27-B6J-VA
Gene Name
Product ID
S-KO-14333
Gene Alias
2610511O17Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ttc27em1/Cya mice (Catalog S-KO-14333) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024882
NCBI RefSeq
NM_152817
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Ttc27, which has a homolog in Caenorhabitis elegans named TRD-1, is a gene encoding a tetratricopeptide repeat-containing protein. In C. elegans, TRD-1 is essential for cell fate determination in the germline and developing epidermis, suggesting Ttc27 may have similar functions in higher organisms [2]. It may also be involved in stem cell-related processes as its ortholog in planarians was found to affect stem cell-associated functions like tissue homeostasis, regeneration, and stem cell maintenance [3].
In a study on Alzheimer-related traits, an association was observed between Ttc27 and white matter hyperintensities (WMH), suggesting a potential role in the genetic basis of Alzheimer disease [1]. Additionally, in mouse brain, the expression of Ttc27 was downregulated in both the cortical and hippocampal regions following chronic constant hypoxia (CCH) and chronic intermittent hypoxia (CIH) [4].
In summary, Ttc27 seems to be involved in cell fate determination, stem cell-related functions, and may play a role in neurodegenerative diseases like Alzheimer disease and response to hypoxic conditions in the brain. Research in model organisms such as C. elegans, planarians, and mice has provided valuable insights into its functions, highlighting its importance in understanding various biological processes and disease mechanisms.
References:
1. Melville, Scott A, Buros, Jacqueline, Parrado, Antonio R, Saykin, Andrew J, Farrer, Lindsay A. 2012. Multiple loci influencing hippocampal degeneration identified by genome scan. In Annals of neurology, 72, 65-75. doi:10.1002/ana.23644. https://pubmed.ncbi.nlm.nih.gov/22745009/
2. Hughes, Samantha, Wilkinson, Henry, Gilbert, Sophie P R, Ding, Siyu Serena, Woollard, Alison. 2014. The C. elegans TPR Containing Protein, TRD-1, Regulates Cell Fate Choice in the Developing Germ Line and Epidermis. In PloS one, 9, e114998. doi:10.1371/journal.pone.0114998. https://pubmed.ncbi.nlm.nih.gov/25493563/
3. Labbé, Roselyne M, Irimia, Manuel, Currie, Ko W, Blencowe, Benjamin J, Pearson, Bret J. . A comparative transcriptomic analysis reveals conserved features of stem cell pluripotency in planarians and mammals. In Stem cells (Dayton, Ohio), 30, 1734-45. doi:10.1002/stem.1144. https://pubmed.ncbi.nlm.nih.gov/22696458/
4. Zhou, Dan, Wang, Jiyi, Zapala, Matthew A, Schork, Nicholas J, Haddad, Gabriel G. 2007. Gene expression in mouse brain following chronic hypoxia: role of sarcospan in glial cell death. In Physiological genomics, 32, 370-9. doi:. https://pubmed.ncbi.nlm.nih.gov/18056785/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen