Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Spsb1em1/Cya
Common Name:
Spsb1-KO
Product ID:
S-KO-14486
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Spsb1-KO
Strain ID
KOCMP-74646-Spsb1-B6J-VA
Gene Name
Spsb1
Product ID
S-KO-14486
Gene Alias
1110014L01Rik; 4930422J18Rik; SSB-1; SSB1
Background
C57BL/6JCya
NCBI ID
74646
Modification
Conventional knockout
Chromosome
4
Phenotype
MGI:1921896
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Spsb1em1/Cya mice (Catalog S-KO-14486) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038562
NCBI RefSeq
NM_029035
Target Region
Exon 2~3
Size of Effective Region
~11.0 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Spsb1, or SPRY domain-containing and SOCS-box protein 1, is an E3 ligase adaptor protein. It is involved in multiple cellular processes and signaling pathways. Spsb1 can interact with various proteins to regulate their stability and function, thereby influencing pathways like the TGF-β signaling pathway, NF-κB activation, and others, which are crucial for processes such as myogenesis, cell survival, and innate immune responses [1,3,8]. Genetic models, especially knockout (KO) mouse models, have been valuable in studying its functions.

In septic mice, increased Spsb1 expression in skeletal muscle was observed. Spsb1 binds to TGF-β receptor-II (TβRII), leading to TβRII ubiquitination and destabilization, which impairs TβRII-Akt-Myogenin signaling, protein synthesis, myoblast fusion, and myogenic differentiation. Down-regulation of Spsb1 by AAV9-mediated shRNA in septic mice attenuated muscle weight loss and atrophy gene expression, indicating its role in sepsis-induced muscle atrophy [1]. In ovarian cancer cells, Spsb1 knockdown decreased cell viability, migration, and elevated p21 levels, suggesting it promotes cell survival by destabilizing p21 [2]. Depletion of Spsb1 in cells resulted in increased NF-κB activation, and over-expression suppressed NF-κB activity, showing its role as a negative regulator of NF-κB activation [3]. In breast cancer, Spsb1 is up-regulated during recurrence, protecting cells from apoptosis and promoting recurrence by potentiating c-MET signaling [4]. Spsb1 and Spsb4 can interact with and facilitate RevErbα ubiquitination and degradation, regulating circadian clock periodicity [5]. In EGF signaling, EGF up-regulates Spsb1, which ubiquitylates hnRNP A1, affecting alternative splicing and cell migration [7]. Spsb1 negatively regulates the TGF-β signaling pathway by enhancing TβRII ubiquitination and degradation [8]. In macrophages, Spsb1 controls the induction of inducible nitric oxide synthase (iNOS) and subsequent NO production downstream of TLR3 and TLR4 [9]. However, Spsb1 deficiency in mice did not affect spermatogenesis or male fertility [6].

In conclusion, Spsb1 plays diverse and essential roles in various biological processes. Its functions range from regulating myogenesis during inflammation, influencing cell survival in cancer, modulating NF-κB activation in innate immunity, to regulating circadian rhythms, alternative splicing, and immune responses. The use of Spsb1 KO mouse models has been instrumental in revealing its role in diseases such as sepsis-induced muscle atrophy and breast cancer recurrence, providing insights into potential therapeutic targets for these conditions.

References:

1. Li, Yi, Dörmann, Niklas, Brinschwitz, Björn, Müller, Oliver J, Fielitz, Jens. 2023. SPSB1-mediated inhibition of TGF-β receptor-II impairs myogenesis in inflammation. In Journal of cachexia, sarcopenia and muscle, 14, 1721-1736. doi:10.1002/jcsm.13252. https://pubmed.ncbi.nlm.nih.gov/37209006/

2. Kim, Hyun-Jung, Kim, Hye Jin, Kim, Mi-Kyung, Kim, Seung Cheol, Ju, Woong. 2019. SPSB1 enhances ovarian cancer cell survival by destabilizing p21. In Biochemical and biophysical research communications, 510, 364-369. doi:10.1016/j.bbrc.2019.01.088. https://pubmed.ncbi.nlm.nih.gov/30712944/

3. Georgana, Iliana, Maluquer de Motes, Carlos. 2020. Cullin-5 Adaptor SPSB1 Controls NF-κB Activation Downstream of Multiple Signaling Pathways. In Frontiers in immunology, 10, 3121. doi:10.3389/fimmu.2019.03121. https://pubmed.ncbi.nlm.nih.gov/32038638/

4. Feng, Yi, Pan, Tien-Chi, Pant, Dhruv K, Ruth, Jason R, Chodosh, Lewis A. 2014. SPSB1 promotes breast cancer recurrence by potentiating c-MET signaling. In Cancer discovery, 4, 790-803. doi:10.1158/2159-8290.CD-13-0548. https://pubmed.ncbi.nlm.nih.gov/24786206/

5. Mekbib, Tsedey, Suen, Ting-Chung, Rollins-Hairston, Aisha, DeBruyne, Jason P. 2019. The E3 Ligases Spsb1 and Spsb4 Regulate RevErbα Degradation and Circadian Period. In Journal of biological rhythms, 34, 610-621. doi:10.1177/0748730419878036. https://pubmed.ncbi.nlm.nih.gov/31607207/

6. Hu, Haoyue, Zhu, Yue, Jiang, Bing, Wu, Yibo, Xi, Xiaoxue. 2025. Testis-enriched Spsb1 is not required for spermatogenesis and fertility in mice. In American journal of translational research, 17, 1824-1833. doi:10.62347/JFJX7128. https://pubmed.ncbi.nlm.nih.gov/40226024/

7. Wang, Feng, Fu, Xing, Chen, Peng, Hu, Ronggui, Hui, Jingyi. 2017. SPSB1-mediated HnRNP A1 ubiquitylation regulates alternative splicing and cell migration in EGF signaling. In Cell research, 27, 540-558. doi:10.1038/cr.2017.7. https://pubmed.ncbi.nlm.nih.gov/28084329/

8. Liu, Sheng, Nheu, Thao, Luwor, Rodney, Nicholson, Sandra E, Zhu, Hong-Jian. 2015. SPSB1, a Novel Negative Regulator of the Transforming Growth Factor-β Signaling Pathway Targeting the Type II Receptor. In The Journal of biological chemistry, 290, 17894-17908. doi:10.1074/jbc.M114.607184. https://pubmed.ncbi.nlm.nih.gov/26032413/

9. Lewis, Rowena S, Kolesnik, Tatiana B, Kuang, Zhihe, Norton, Raymond S, Nicholson, Sandra E. 2011. TLR regulation of SPSB1 controls inducible nitric oxide synthase induction. In Journal of immunology (Baltimore, Md. : 1950), 187, 3798-805. doi:10.4049/jimmunol.1002993. https://pubmed.ncbi.nlm.nih.gov/21876038/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest