C57BL/6JCya-Bcdin3dem1/Cya
Common Name:
Bcdin3d-KO
Product ID:
S-KO-14595
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Contact for Pricing
Basic Information
Strain Name
Bcdin3d-KO
Strain ID
KOCMP-75284-Bcdin3d-B6J-VA
Gene Name
Product ID
S-KO-14595
Gene Alias
4930556P03Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bcdin3dem1/Cya mice (Catalog S-KO-14595) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040313
NCBI RefSeq
NM_029236
Target Region
Exon 1~2
Size of Effective Region
~4.2 kb
Detailed Document
Overview of Gene Research
BCDIN3D, a member of the Bin3 methyltransferase family, is an RNA phospho-methyltransferase conserved from worm to human [1]. It methylates the 5' monophosphate of specific RNAs, including cytoplasmic tRNAHis and certain precursor microRNAs [1,2,4,6]. This methylation can regulate RNA processing and function, potentially influencing various biological processes such as miRNA biogenesis, metabolism, and cell proliferation [2,6]. Genetic models, like Drosophila ortholog studies, can be valuable in further understanding its functions [5].
In Drosophila, ovary-specific knockdown of the BCDIN3D ortholog causes female sterility and dysregulates miRNA and mRNA profiles, with many affected genes involved in metabolic processes, ribonucleoprotein complex regulation, and translational control [5]. In human breast cancer cells, BCDIN3D depletion leads to increased levels of Fructose 1,6 Bisphosphate due to down-regulation of Aldolase C, and it regulates Aldolase C through a let-7 microRNA-related mechanism [2]. Also, in cervical cancer cells, miR-195-3p inhibits cell proliferation by targeting BCDIN3D [3].
Overall, BCDIN3D plays crucial roles in RNA processing, especially of tRNAHis and miRNAs, and is involved in biological processes like female fertility in Drosophila. In human cancers, such as breast and cervical cancer, BCDIN3D is associated with disease-related phenotypes. Studies using gene knockdown models in different organisms have revealed its functions in specific biological processes and disease-related pathways, highlighting its potential as a therapeutic target.
References:
1. Tomita, Kozo, Liu, Yining. 2018. Human BCDIN3D Is a Cytoplasmic tRNAHis-Specific 5'-Monophosphate Methyltransferase. In Frontiers in genetics, 9, 305. doi:10.3389/fgene.2018.00305. https://pubmed.ncbi.nlm.nih.gov/30127802/
2. Reinsborough, Calder W, Ipas, Hélène, Abell, Nathan S, Van Den Berg, Carla, Xhemalçe, Blerta. 2021. BCDIN3D RNA methyltransferase stimulates Aldolase C expression and glycolysis through let-7 microRNA in breast cancer cells. In Oncogene, 40, 2395-2406. doi:10.1038/s41388-021-01702-y. https://pubmed.ncbi.nlm.nih.gov/33664453/
3. Jin, Minfei, Wang, Lei, Zheng, Tao, Sheng, Rong, Zhu, Hong. 2020. MiR-195-3p inhibits cell proliferation in cervical cancer by targeting BCDIN3D. In Journal of reproductive immunology, 143, 103211. doi:10.1016/j.jri.2020.103211. https://pubmed.ncbi.nlm.nih.gov/33157501/
4. Martinez, Anna, Yamashita, Seisuke, Nagaike, Takashi, Suzuki, Tsutomu, Tomita, Kozo. . Human BCDIN3D monomethylates cytoplasmic histidine transfer RNA. In Nucleic acids research, 45, 5423-5436. doi:10.1093/nar/gkx051. https://pubmed.ncbi.nlm.nih.gov/28119416/
5. Zhu, Li, Liao, Susan E, Ai, Yiwei, Fukunaga, Ryuya. 2019. RNA methyltransferase BCDIN3D is crucial for female fertility and miRNA and mRNA profiles in Drosophila ovaries. In PloS one, 14, e0217603. doi:10.1371/journal.pone.0217603. https://pubmed.ncbi.nlm.nih.gov/31145769/
6. Xhemalce, Blerta, Robson, Samuel C, Kouzarides, Tony. . Human RNA methyltransferase BCDIN3D regulates microRNA processing. In Cell, 151, 278-88. doi:10.1016/j.cell.2012.08.041. https://pubmed.ncbi.nlm.nih.gov/23063121/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen