C57BL/6JCya-Ccdc7bem1/Cya
Common Name
Ccdc7b-KO
Product ID
S-KO-14633
Backgroud
C57BL/6JCya
Strain ID
KOCMP-75453-Ccdc7b-B6J-VA
Status
When using this mouse strain in a publication, please cite “Ccdc7b-KO Mouse (Catalog S-KO-14633) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ccdc7b-KO
Strain ID
KOCMP-75453-Ccdc7b-B6J-VA
Gene Name
Product ID
S-KO-14633
Gene Alias
1700008F21Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 8
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000026912
NCBI RefSeq
NM_001168369
Target Region
Exon 3~14
Size of Effective Region
~71.3 kb
Overview of Gene Research
Ccdc7b, also known as Biot2-L, contains a conserved domain-Ccdc7. It is highly expressed in adult testis, suggesting its involvement in testis development and spermatogonia [3].
RNA interference against Biot2 (a related gene, potentially an alias) inhibits the growth of tumor cells in vitro and in vivo. Treating B16 murine melanoma, LL/2 murine Lewis lung carcinoma and CT26 murine colorectal adenocarcinoma with special shRNA targeting Biot2 significantly reduces their proliferation rate in vitro [1]. Silencing Biot2 in CT26 cells by RNA interference also induces G1 phase arrest and apoptosis through regulation of p16 and p21 [2].
In conclusion, Ccdc7b (Biot2-L) seems to play a role in testis-related biological processes. The related gene Biot2 shows potential as a target for biotherapy and gene therapy in cancer, as demonstrated by RNA interference studies, which help in understanding its function in tumorigenesis.
References:
1. Wang, Chun-Ting, Zhang, Peng, Wang, Yong-Sheng, Yang, Han-Shuo, Wei, Yu-Quan. 2009. RNA interference against Biot2, a novel mouse testis-specific gene, inhibits the growth of tumor cells. In Cellular & molecular biology letters, 14, 363-76. doi:10.2478/s11658-009-0004-6. https://pubmed.ncbi.nlm.nih.gov/19277478/
2. Zhou, Cong, Zhang, Peng, Xu, Guang-Chao, Zeng, Qi, Wang, Chun-Ting. . RNA interference of Biot2 induces G1 phase arrest and apoptosis in mouse colorectal cancer cell line. In Oncology research, 22, 93-103. doi:10.3727/096504014X14146137738583. https://pubmed.ncbi.nlm.nih.gov/25706396/
3. Wang, Hao, Zhang, Peng, Wang, Chun-Ting. . [Analysis of expression pattern of a novel testis-highly expressed gene Biot2-L and the primary study on its role in testis development]. In Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 40, 853-6. doi:. https://pubmed.ncbi.nlm.nih.gov/19950598/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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