C57BL/6JCya-Cox8cem1/Cya
Common Name
Cox8c-KO
Product ID
S-KO-14644
Backgroud
C57BL/6JCya
Strain ID
KOCMP-75483-Cox8c-B6J-VA
When using this mouse strain in a publication, please cite “Cox8c-KO Mouse (Catalog S-KO-14644) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Cox8c-KO
Strain ID
KOCMP-75483-Cox8c-B6J-VA
Gene Name
Product ID
S-KO-14644
Gene Alias
1700007F21Rik, COX8-3, COXVIII-3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 12
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000053611
NCBI RefSeq
NM_001039049
Target Region
Exon 1~2
Size of Effective Region
~0.9 kb
Overview of Gene Research
Cox8c is a gene associated with the metabolic disorders of the nervous system [1]. It is a testis-enriched cytochrome c oxidase (COX) subunit. COX is an oligomeric complex in the mitochondrial inner membrane and the terminal enzyme of the mitochondrial electron transport chain in eukaryotes, involved in the production of ATP through oxidative phosphorylation [2].
In gene-knockout studies, disrupting Cox8c in mice does not affect male fertility, indicating that this testis-enriched COX subunit is not essential for oxidative phosphorylation in mouse spermatozoa [2]. Additionally, in a genetic screen of somatic cell nuclear transfer-reprogramming resistant genes, Cox8c was found to be exclusively expressed in the testis, but its knockout did not lead to abnormal sperm production compared to other genes in the study [3]. In patients with tethered spinal cord syndrome, chromosomal aberration of Cox8c was detected, suggesting its potential association with the pathogenesis of this disease [1].
In conclusion, Cox8c, as a testis-enriched COX subunit, does not seem to be essential for sperm-related OXPHOS and male fertility based on mouse KO models. Its chromosomal aberration may be related to tethered spinal cord syndrome, providing insights into understanding the gene's function in nervous system-related metabolic disorders and male germ cell development.
References:
1. Zhao, Qiu-Jiong, Bai, Shao-Cong, Cheng, Cheng, Hang, Xing-Yi, Shang, Ai-Jia. . Association between chromosomal aberration of COX8C and tethered spinal cord syndrome: array-based comparative genomic hybridization analysis. In Neural regeneration research, 11, 1333-8. doi:10.4103/1673-5374.189200. https://pubmed.ncbi.nlm.nih.gov/27651783/
2. Shimada, Keisuke, Lu, Yonggang, Ikawa, Masahito. 2023. Disruption of testis-enriched cytochrome c oxidase subunit COX6B2 but not COX8C leads to subfertility. In Experimental animals, 73, 1-10. doi:10.1538/expanim.23-0055. https://pubmed.ncbi.nlm.nih.gov/37423748/
3. Akter, Most Sumona, Hada, Masashi, Shikata, Daiki, Ogura, Atsuo, Matoba, Shogo. 2021. CRISPR/Cas9-based genetic screen of SCNT-reprogramming resistant genes identifies critical genes for male germ cell development in mice. In Scientific reports, 11, 15438. doi:10.1038/s41598-021-94851-9. https://pubmed.ncbi.nlm.nih.gov/34326397/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.