C57BL/6JCya-Morn5em1/Cya
Common Name:
Morn5-KO
Product ID:
S-KO-14646
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Morn5-KO
Strain ID
KOCMP-75495-Morn5-B6J-VA
Gene Name
Product ID
S-KO-14646
Gene Alias
1700010A17Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Morn5em1/Cya mice (Catalog S-KO-14646) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028256
NCBI RefSeq
NM_029309
Target Region
Exon 2~4
Size of Effective Region
~4.2 kb
Detailed Document
Overview of Gene Research
MORN5, or MORN repeat containing 5, has the MORN motif found in multiple-copy proteins across animal and plant kingdoms. It is involved in craniofacial development and may interact with the BMP and TGFβ pathways. Its expression in craniofacial structures could be crucial for normal development, and its study in genetic models may help understand its functions [1].
In chicken embryos, MORN5 expression was first seen at stage HH17-18 (E2.5) on either side of the primitive oral cavity. From stage HH20 to HH29 (E3-E6), there was strong expression in the maxillary and mandibular prominences. MORN5 was downregulated after Noggin and/or RA treatment, and BMP pathway genes were downstream of MORN5 following siRNA knockdown. Also, in mice, MORN5 is conserved between mouse and human sperm and is associated with cytoskeletal structures in the sperm flagellum [1,2].
In conclusion, MORN5 plays important roles in craniofacial development and sperm function. Its regulation of BMP signaling in craniofacial development, as seen in the chicken model, and its association with sperm-tail cytoskeletal structures in mice, contribute to understanding these biological processes. These findings may be relevant to human non-syndromic cleft lip with or without cleft palate, based on the association of MORN5 variants with increased risk [1].
References:
1. Cela, Petra, Hampl, Marek, Fu, Katherine K, Richman, Joy M, Buchtova, Marcela. 2016. MORN5 Expression during Craniofacial Development and Its Interaction with the BMP and TGFβ Pathways. In Frontiers in physiology, 7, 378. doi:10.3389/fphys.2016.00378. https://pubmed.ncbi.nlm.nih.gov/27630576/
2. Kwon, Jun Tae, Ham, Sera, Jeon, Suyeon, Oh, Seungmin, Cho, Chunghee. 2017. Expression of uncharacterized male germ cell-specific genes and discovery of novel sperm-tail proteins in mice. In PloS one, 12, e0182038. doi:10.1371/journal.pone.0182038. https://pubmed.ncbi.nlm.nih.gov/28742876/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen