C57BL/6JCya-Dcstampem1/Cya
Common Name:
Dcstamp-KO
Product ID:
S-KO-14737
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dcstamp-KO
Strain ID
KOCMP-75766-Dcstamp-B6J-VA
Gene Name
Product ID
S-KO-14737
Gene Alias
4833414I07Rik; DC-STAMP; FIND; Tm7sf4; mDC-STAMP
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dcstampem1/Cya mice (Catalog S-KO-14737) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022913
NCBI RefSeq
NM_029422
Target Region
Exon 2~4
Size of Effective Region
~6.1 kb
Detailed Document
Overview of Gene Research
DCSTAMP, also known as TM7SF4, is a gene encoding dendritic cell-specific transmembrane protein. It plays a crucial role in osteoclast differentiation, especially in the cell-cell fusion process, which is essential for the formation of multinucleated osteoclasts. The gene is associated with pathways such as NF-κB and NFATc1, which are also involved in osteoclast-related functions [1,3,4]. Understanding DCSTAMP is of great significance for studying bone-related biological processes and diseases.
In multiple studies, substances like aconine, artesunate, and matrix-bound nanovesicles have been shown to inhibit osteoclast differentiation and activity by suppressing DC-STAMP expression. For example, aconine inhibits RANKL-induced osteoclast differentiation in RAW264.7 cells by suppressing NF-κB and NFATc1 activation and DC-STAMP expression [1]. Artesunate attenuates LPS-induced osteoclastogenesis by suppressing TLR4/TRAF6 and PLCγ1-Ca2 +-NFATc1 signaling pathway, along with inhibiting DC-STAMP expression [3]. Matrix-bound nanovesicles alleviate particulate-induced periprosthetic osteolysis by suppressing the NF-κB signaling pathway and downstream DC-STAMP expression [4]. Also, in a study on familial Paget's disease of bone, a c.1189C>T p.L397F variant in DC-STAMP was identified, but it was concluded that genetic variation in DCSTAMP is not a significant predisposing factor in that specific cohort [2].
In conclusion, DCSTAMP is a key regulator in osteoclast differentiation, particularly in cell-cell fusion. Research on substances affecting DC-STAMP expression and genetic variants in DCSTAMP has provided insights into its role in bone-related diseases such as osteoclast-mediated bone resorption disorders and Paget's disease of bone. These findings contribute to understanding the biological mechanisms underlying these diseases and may potentially lead to new therapeutic strategies.
References:
1. Zeng, Xiang-zhou, He, Long-gang, Wang, Song, Li, Xiao-juan, Liu, Shu-wen. 2015. Aconine inhibits RANKL-induced osteoclast differentiation in RAW264.7 cells by suppressing NF-κB and NFATc1 activation and DC-STAMP expression. In Acta pharmacologica Sinica, 37, 255-63. doi:10.1038/aps.2015.85. https://pubmed.ncbi.nlm.nih.gov/26592521/
2. Sultana, M A, Pavlos, N J, Ward, Lynley, Walsh, J P, Rea, S L. 2019. Targeted sequencing of DCSTAMP in familial Paget's disease of bone. In Bone reports, 10, 100198. doi:10.1016/j.bonr.2019.100198. https://pubmed.ncbi.nlm.nih.gov/30886882/
3. Zeng, Xiang-Zhou, Zhang, Yue-Yang, Yang, Qin, Liu, Shu-Wen, Li, Xiao-Juan. 2019. Artesunate attenuates LPS-induced osteoclastogenesis by suppressing TLR4/TRAF6 and PLCγ1-Ca2+-NFATc1 signaling pathway. In Acta pharmacologica Sinica, 41, 229-236. doi:10.1038/s41401-019-0289-6. https://pubmed.ncbi.nlm.nih.gov/31431733/
4. Liao, Runzhi, Dewey, Marley J, Rong, Jiayang, Hussey, George S, Badylak, Stephen F. 2024. Matrix-bound nanovesicles alleviate particulate-induced periprosthetic osteolysis. In Science advances, 10, eadn1852. doi:10.1126/sciadv.adn1852. https://pubmed.ncbi.nlm.nih.gov/39423278/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen