C57BL/6JCya-Fads1em1/Cya
Common Name:
Fads1-KO
Product ID:
S-KO-14821
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Fads1-KO
Strain ID
KOCMP-76267-Fads1-B6J-VA
Gene Name
Product ID
S-KO-14821
Gene Alias
0710001O03Rik; A930006B21Rik; DSD
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fads1em1/Cya mice (Catalog S-KO-14821) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000010807
NCBI RefSeq
NM_146094
Target Region
Exon 1~2
Size of Effective Region
~3.4 kb
Detailed Document
Overview of Gene Research
Fads1, also known as delta-5 desaturase, is a rate-limiting enzyme in long-chain polyunsaturated fatty acid (LCPUFA) synthesis. It catalyzes the conversion of dihomo-gamma-linolenic acid (DGLA) to arachidonic acid (AA), playing a crucial role in fatty acid metabolism. This process is involved in various biological pathways, such as the synthesis of prostaglandin E2 (PGE2) via the TLR4/MYD88 pathway in colorectal cancer cells, and is also relevant to lipid metabolism and ferroptosis susceptibility in triple-negative breast cancer [1,2].
In colorectal cancer, Fads1-arachidonic acid axis promotes tumor growth by altering the intestinal microecology to increase gram-negative bacteria. The elimination of gram-negative microbes abolishes the effect of Fads1 [1]. In triple-negative breast cancer, Fads1/2 high-expressing tumors are susceptible to ferroptosis-inducing agents, and targeting Fads1/2 can render tumors ferroptosis-resistant [2]. In laryngeal squamous cell carcinoma, Fads1 promotes cancer cell proliferation, migration, and invasion by activating the AKT/mTOR signaling pathway [3]. In bladder cancer, high Fads1 expression is associated with advanced clinicopathological features and poor prognosis [4].
In conclusion, Fads1 is essential for fatty acid metabolism and is involved in the development and progression of multiple cancers, including colorectal, triple-negative breast, laryngeal squamous cell, and bladder cancers. The study of Fads1 using in vivo models like gene knockout or conditional knockout mouse models (although not explicitly detailed in all references here) could potentially provide more insights into its role in these disease conditions, helping to understand the underlying mechanisms and potentially develop new therapeutic strategies.
References:
1. Xu, Chunjie, Gu, Lei, Hu, Lipeng, Zhang, Xueli, Xu, Qing. 2023. FADS1-arachidonic acid axis enhances arachidonic acid metabolism by altering intestinal microecology in colorectal cancer. In Nature communications, 14, 2042. doi:10.1038/s41467-023-37590-x. https://pubmed.ncbi.nlm.nih.gov/37041160/
2. Lorito, Nicla, Subbiani, Angela, Smiriglia, Alfredo, Bachi, Angela, Morandi, Andrea. 2024. FADS1/2 control lipid metabolism and ferroptosis susceptibility in triple-negative breast cancer. In EMBO molecular medicine, 16, 1533-1559. doi:10.1038/s44321-024-00090-6. https://pubmed.ncbi.nlm.nih.gov/38926633/
3. Zhao, Rui, Tian, Linli, Zhao, Bo, Shang, Desi, Liu, Ming. 2020. FADS1 promotes the progression of laryngeal squamous cell carcinoma through activating AKT/mTOR signaling. In Cell death & disease, 11, 272. doi:10.1038/s41419-020-2457-5. https://pubmed.ncbi.nlm.nih.gov/32332698/
4. Lu, Ying, Shao, Jing, Shu, Xu, Lai, Yongkang, Liu, Ji. . FADS1 is a Prognostic Biomarker in Bladder Cancer: A Study Based on TCGA Data. In Combinatorial chemistry & high throughput screening, 24, 1197-1204. doi:10.2174/1386207323666200925104911. https://pubmed.ncbi.nlm.nih.gov/32981498/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen