C57BL/6JCya-Nkrfem1/Cya
Common Name
Nkrf-KO
Product ID
S-KO-15035
Backgroud
C57BL/6JCya
Strain ID
KOCMP-77286-Nkrf-B6J-VA
When using this mouse strain in a publication, please cite “Nkrf-KO Mouse (Catalog S-KO-15035) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Nkrf-KO
Strain ID
KOCMP-77286-Nkrf-B6J-VA
Gene Name
Product ID
S-KO-15035
Gene Alias
9430034D17Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr X
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000239259
NCBI RefSeq
NM_029891
Target Region
Exon 2
Size of Effective Region
~1.8 kb
Overview of Gene Research
NKRF, also known as NF-κB-repressing factor, plays a significant role in the transcriptional inhibition of various specific genes. It is closely associated with the NF-κB signaling pathway, which is crucial in regulating immune responses, inflammation, and cell survival. The study of NKRF using gene knockout models, such as KO or CKO mouse models, has been instrumental in understanding its functions in different biological processes and disease conditions [1,2].
In a CF-specific NKRF-knockout (NKRF-CKO) mouse model, NKRF was found to protect against cardiac remodeling post-myocardial infarction. NKRF expression was downregulated in CFs during pathological cardiac remodeling in vivo and in response to TNF-α in vitro. NKRF-CKO mice showed worse cardiac function, increased infarct size, heart weight, and MMP2 and MMP9 expression compared to littermates. Mechanistically, NKRF inhibits human antigen R (HuR) transcription, decreasing HuR-targeted Mmp2 and Mmp9 mRNA stability [1]. Meta-analysis and experimental validations in mouse models also showed that NKRF and ZBTB17 regulate the NF-κB signaling pathway, contributing to the shared molecular mechanisms of Alzheimer's disease and atherosclerosis [2].
In conclusion, NKRF is an important regulator in the NF-κB signaling pathway. Studies using NKRF KO/CKO mouse models have revealed its protective role in cardiac remodeling post-myocardial infarction and its contribution to the shared molecular mechanisms of Alzheimer's disease and atherosclerosis. These findings highlight the significance of NKRF in understanding the pathogenesis of these diseases and potentially developing targeted therapies.
References:
1. Guo, Chenghu, Ji, Wei, Yang, Wei, Zhang, Meng, Zhang, Cheng. 2023. NKRF in Cardiac Fibroblasts Protects against Cardiac Remodeling Post-Myocardial Infarction via Human Antigen R. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2303283. doi:10.1002/advs.202303283. https://pubmed.ncbi.nlm.nih.gov/37667861/
2. Zhang, Di, Chen, Keyan, Shan, Li Shen. . Meta-analysis and transcriptomic analysis reveal that NKRF and ZBTB17 regulate the NF-κB signaling pathway, contributing to the shared molecular mechanisms of Alzheimer's disease and atherosclerosis. In CNS neuroscience & therapeutics, 30, e14683. doi:10.1111/cns.14683. https://pubmed.ncbi.nlm.nih.gov/38738952/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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