C57BL/6JCya-Map7d2em1/Cya
Common Name:
Map7d2-KO
Product ID:
S-KO-15151
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Map7d2-KO
Strain ID
KOCMP-78283-Map7d2-B6J-VA
Gene Name
Product ID
S-KO-15151
Gene Alias
1600028E09Rik; 2900002G04Rik; 5330432J06Rik; C030036K04Rik; Mtap7d2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Map7d2em1/Cya mice (Catalog S-KO-15151) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000112471
NCBI RefSeq
NM_001081124
Target Region
Exon 4~6
Size of Effective Region
~4.7 kb
Detailed Document
Overview of Gene Research
Map7d2, a member of the MAP7 family, is a microtubule-associated protein. It plays a crucial role in modulating microtubule (MT) dynamics, which are essential for various cellular processes such as cell motility, neurite outgrowth, and intracellular cargo transport. It is involved in pathways related to the regulation of MT-based functions and may have broader implications for normal cellular development and function [2,3].
In colorectal cancer (CRC) patients with microsatellite stable (MSS) tumors, Map7d2 has been shown to reduce CD8+ cytotoxic T lymphocyte (CTL) infiltration. Knockdown of Map7d2 in in vitro and in vivo experiments significantly increased the infiltration of CD8+ CTLs, inhibiting tumor progression and improving the efficacy of immune checkpoint inhibitors in MSS CRC murine models. Mechanistically, Map7d2 interacts with MYH9, protecting it from ubiquitin-mediated degradation, which in turn decreases the secretion of HMGB1 that suppresses CD8+ CTL infiltration [1]. In neuronal cells, loss of Map7d2 led to decreased resistance to the MT-destabilizing agent nocodazole and increased random cell migration and neurite outgrowth, suggesting its role in MT stabilization [2]. Depletion of Map7d2 in neurons resulted in reduced axonal cargo entry and defects in axon development and neuronal migration, indicating its function in promoting kinesin-1-mediated cargo transport into the axon [3].
In summary, Map7d2 is vital for microtubule-related functions in normal cell processes. Its role in cancer, especially in regulating immune cell infiltration in colorectal cancer, is significant. Studies using gene knockdown (functionally similar to gene knockout in revealing gene function) in in vitro and in vivo models have provided insights into its functions, highlighting its potential as a therapeutic target in CRC and its importance in understanding neuronal development and function.
References:
1. Wu, Qian, Yue, Xiao, Liu, Huashan, Lan, Ping, Wu, Xianrui. 2022. MAP7D2 reduces CD8+ cytotoxic T lymphocyte infiltration through MYH9-HMGB1 axis in colorectal cancer. In Molecular therapy : the journal of the American Society of Gene Therapy, 31, 90-104. doi:10.1016/j.ymthe.2022.09.001. https://pubmed.ncbi.nlm.nih.gov/36081350/
2. Kikuchi, Koji, Sakamoto, Yasuhisa, Uezu, Akiyoshi, Hisanaga, Shin-Ichi, Nakanishi, Hiroyuki. 2022. Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cells. In Life science alliance, 5, . doi:10.26508/lsa.202201390. https://pubmed.ncbi.nlm.nih.gov/35470240/
3. Pan, Xingxiu, Cao, Yujie, Stucchi, Riccardo, Harterink, Martin, Hoogenraad, Casper C. . MAP7D2 Localizes to the Proximal Axon and Locally Promotes Kinesin-1-Mediated Cargo Transport into the Axon. In Cell reports, 26, 1988-1999.e6. doi:10.1016/j.celrep.2019.01.084. https://pubmed.ncbi.nlm.nih.gov/30784582/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen