C57BL/6JCya-Pym1em1/Cya
Common Name:
Pym1-KO
Product ID:
S-KO-15173
Background:
C57BL/6JCya
Product Type
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Basic Information
Strain Name
Pym1-KO
Strain ID
KOCMP-78428-Pym1-B6J-VA
Gene Name
Product ID
S-KO-15173
Gene Alias
A030010B05Rik; Pym; Wibg
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pym1em1/Cya mice (Catalog S-KO-15173) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000163377
NCBI RefSeq
NM_030100
Target Region
Exon 2~3
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
PYM1, also known as PYM, is an important factor involved in the regulation of mRNA fate. It can bind both the ribosome and the RBM8A/MAGOH heterodimer of the Exon Junction Complex (EJC) core. EJC, deposited upstream of exon-exon junctions during pre-mRNA splicing, modulates mRNA fate at multiple post-transcriptional steps, and PYM1 is involved in an EJC disassembly mechanism, which is crucial for translation-independent EJC destabilization [1].
In human embryonic kidney 293 (HEK293) cells, acute reduction of PYM1 levels modestly inhibits nonsense-mediated mRNA decay and stabilizes certain mRNAs. These mRNAs localize to endoplasmic reticulum-associated TIS-granules and are characterized by fewer and longer exons [1]. Also, in flaviviral infections, PYM1 has antiviral roles. Flavivirus capsids interact with PYM1, and depletion of PYM1 attenuates RBM8A binding to viral RNA, suggesting that the virus sequesters PYM1 to protect viral RNA from decay [2].
In summary, PYM1 is a key factor in regulating mRNA fate through its role in EJC disassembly. Its function has implications in processes like nonsense-mediated mRNA decay and in antiviral defense mechanisms, especially in the context of flaviviral infections. Studies on PYM1 help to understand the complex interplay between host cells and viruses at the post-transcriptional regulation level.
References:
1. Sanjeev, Manu, Woodward, Lauren A, Schiff, Michael L, Bundschuh, Ralf, Singh, Guramrit. 2025. PYM1 limits non-canonical Exon Junction Complex occupancy in a gene architecture dependent manner to tune mRNA expression. In bioRxiv : the preprint server for biology, , . doi:10.1101/2025.03.13.643037. https://pubmed.ncbi.nlm.nih.gov/40161626/
2. Li, Minghua, Johnson, Jeffrey R, Truong, Billy, Cherry, Sara, Ramage, Holly. 2019. Identification of antiviral roles for the exon-junction complex and nonsense-mediated decay in flaviviral infection. In Nature microbiology, 4, 985-995. doi:10.1038/s41564-019-0375-z. https://pubmed.ncbi.nlm.nih.gov/30833725/
3. Gehring, Niels H, Lamprinaki, Styliani, Kulozik, Andreas E, Hentze, Matthias W. . Disassembly of exon junction complexes by PYM. In Cell, 137, 536-48. doi:10.1016/j.cell.2009.02.042. https://pubmed.ncbi.nlm.nih.gov/19410547/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen