C57BL/6JCya-Vsig1em1/Cya
Common Name:
Vsig1-KO
Product ID:
S-KO-15223
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Vsig1-KO
Strain ID
KOCMP-78789-Vsig1-B6J-VA
Gene Name
Product ID
S-KO-15223
Gene Alias
1700062D20Rik; 4930405J24Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Vsig1em1/Cya mice (Catalog S-KO-15223) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033806
NCBI RefSeq
NM_030181
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Vsig1, or V-set and immunoglobulin domain containing 1, is a cell-cell adhesion molecule. It belongs to the immunoglobulin superfamily and is preferentially expressed in the stomach, testis, and certain cancers such as gastric, esophageal, and ovarian cancers [3]. Functionally, it may be involved in cell-cell adhesion processes and is hypothesized to play a role in the epithelial-mesenchymal transition (EMT) phenomenon. Genetic models, like gene knockout (KO) mice, can be valuable in studying its function.
In mice, knocking out Vsig1 using Nuclease technology-mediated genome editing showed that Vsig1 may not be essential for spermatogenesis and normal testis development, as male KO mice had normal sperm number and motility and were fertile [2]. In gastric cancer, the membrane-cytoplasmic translocation of VSIG1 can indicate EMT due to the activation of the canonical Wnt/β-catenin signaling pathway. Also, cases with subcellular translocation of VSIG1 had the poorest overall survival. The loss of VSIG1 expression was significantly correlated with poor overall survival and disease-free survival, suggesting it may serve as a prognostic marker and a potential therapeutic target for gastric cancer [1,4].
In conclusion, Vsig1 has an important role in the differentiation of glandular gastric epithelia and is associated with the development and prognosis of gastric cancer. The use of Vsig1 KO mouse models has provided insights into its non-essential role in spermatogenesis and its significance in gastric cancer, helping to understand the biological functions and disease-related mechanisms associated with Vsig1.
References:
1. Satala, Catalin-Bogdan, Jung, Ioan, Kovacs, Zsolt, Patrichi, Andrei-Ionut, Gurzu, Simona. 2022. V-set and immunoglobulin domain containing 1 (VSIG1) as an emerging target for epithelial-mesenchymal transition of gastric cancer. In Scientific reports, 12, 16241. doi:10.1038/s41598-022-19883-1. https://pubmed.ncbi.nlm.nih.gov/36171238/
2. Jung, Yena, Bang, Hyewon, Kim, Young-Hyun, Kim, Sun-Uk, Kim, Ekyune. 2021. V-Set and Immunoglobulin Domain-Containing 1 (VSIG1), Predominantly Expressed in Testicular Germ Cells, Is Dispensable for Spermatogenesis and Male Fertility in Mice. In Animals : an open access journal from MDPI, 11, . doi:10.3390/ani11041037. https://pubmed.ncbi.nlm.nih.gov/33916888/
3. Oidovsambuu, Odgerel, Nyamsuren, Gunsmaa, Liu, Shuai, Engel, Wolfgang, Adham, Ibrahim M. 2011. Adhesion protein VSIG1 is required for the proper differentiation of glandular gastric epithelia. In PloS one, 6, e25908. doi:10.1371/journal.pone.0025908. https://pubmed.ncbi.nlm.nih.gov/21991385/
4. Chen, Yibing, Pan, Ke, Li, Shengping, He, Jia, Li, Qiao. 2011. Decreased expression of V-set and immunoglobulin domain containing 1 (VSIG1) is associated with poor prognosis in primary gastric cancer. In Journal of surgical oncology, 106, 286-93. doi:10.1002/jso.22150. https://pubmed.ncbi.nlm.nih.gov/22095633/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen