C57BL/6NCya-Wsb1em1/Cya
Common Name:
Wsb1-KO
Product ID:
S-KO-15240
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Wsb1-KO
Strain ID
KOCMP-78889-Wsb1-B6N-VA
Gene Name
Product ID
S-KO-15240
Gene Alias
1110056B13Rik; 2700038M07Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Wsb1em1/Cya mice (Catalog S-KO-15240) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000017821
NCBI RefSeq
NM_019653
Target Region
Exon 2~7
Size of Effective Region
~9.6 kb
Detailed Document
Overview of Gene Research
Wsb1, also known as WD repeat and suppressor of cytokine signaling box containing 1, is an E3 ubiquitin ligase. It functions as a substrate recognition protein within an E3 ubiquitin ligase complex, mediating ubiquitinylation and proteolytic degradation of diverse targets. It is involved in multiple pathways such as β -catenin signaling, NF -κB regulation, and is associated with processes like thyroid hormone homeostasis, immune regulation, glucose metabolism, and hypoxia [2]. It has significance in developmental biology and is dysregulated in several types of cancer, thus being potentially crucial for understanding tumorigenesis and cancer progression.
In cancer, Wsb1 has been found to form a feedforward circuit with c -Myc. Wsb1 is a direct target gene of c -Myc and positively regulates c -Myc expression through the β -catenin pathway, promoting cancer development [1]. In prostate cancer, increased Wsb1 expression is associated with higher grades and worse prognosis, and it has a central role in influencing other key genes like EZH1, EZH2, WNT10a, and WNT10b [3]. In lung fibrosis, conditional deletion of Wsb1 in adult mice ameliorated bleomycin-induced pulmonary fibrosis. Wsb1-deficient mice showed reduced lipofibroblast to myofibroblast transition but enhanced alveolar type 2 cell proliferation and differentiation into alveolar type 1 cells, suggesting Wsb1's role in myofibroblast accumulation and alveolar epithelial regeneration [4]. In myocardial ischemia-reperfusion injury, overexpression of Wsb1 relieved injury, as it activated β -catenin signaling by promoting GSK3β ubiquitination [5].
In conclusion, Wsb1 is an E3 ubiquitin ligase with diverse functions in various biological processes and disease conditions. Gene knockout and conditional knockout mouse models have been instrumental in revealing its roles in cancer progression, lung fibrosis, and myocardial ischemia-reperfusion injury, providing insights into potential therapeutic targets for these diseases.
References:
1. Gao, Xiaomeng, You, Jieqiong, Gong, Yanling, Yang, Bo, Cao, Ji. 2021. WSB1 regulates c-Myc expression through β-catenin signaling and forms a feedforward circuit. In Acta pharmaceutica Sinica. B, 12, 1225-1239. doi:10.1016/j.apsb.2021.10.021. https://pubmed.ncbi.nlm.nih.gov/35530152/
2. Haque, Moinul, Kendal, Joseph Keith, MacIsaac, Ryan Matthew, Demetrick, Douglas James. 2016. WSB1: from homeostasis to hypoxia. In Journal of biomedical science, 23, 61. doi:10.1186/s12929-016-0270-3. https://pubmed.ncbi.nlm.nih.gov/27542736/
3. Boldrini, Laura, Bardi, Massimo. 2023. WSB1 Involvement in Prostate Cancer Progression. In Genes, 14, . doi:10.3390/genes14081558. https://pubmed.ncbi.nlm.nih.gov/37628609/
4. Chong, Lei, Zou, Lihui, Xiang, Liyan, Zhang, Hailin, Zhang, Jin-San. 2024. WSB1, a Hypoxia-Inducible E3 Ligase, Promotes Myofibroblast Accumulation and Attenuates Alveolar Epithelial Regeneration in Mouse Lung Fibrosis. In The American journal of pathology, 194, 656-672. doi:10.1016/j.ajpath.2024.01.010. https://pubmed.ncbi.nlm.nih.gov/38325552/
5. Fang, Lini, Tao, Yang, Che, Guoying, Ren, Min, Liu, Yujie. 2024. WSB1, as an E3 ligase, restrains myocardial ischemia-reperfusion injury by activating β-catenin signaling via promoting GSK3β ubiquitination. In Molecular medicine (Cambridge, Mass.), 30, 31. doi:10.1186/s10020-024-00800-3. https://pubmed.ncbi.nlm.nih.gov/38395742/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen